8个彩色冬小麦品种农艺性状调查及ROS信号转导机理研究  

Investigation on Agronomic Traits and Study on ROS Signal Transduction Mechanism of 8 Colored Winter Wheat Varieties

作　　者：李娜 郑琪 姚来来 祁伟亮 倪胜利[3] 刘愈之 王亚翠 刘众 郭怀亮 张旭辉 马强强 孔丽婷 李兴茂[3] LI Na;ZHENG Qi;YAO Lailai;QI Weiliang;NI Shengli;LIU Yuzhi;WANG Yacui;LIU Zhong;GUO Huailiang;ZHANG Xuhui;MA Qiangqiang;KONG Liting;LI Xingmao(Pingliang Academy of Agricultural Sciences,Pingliang Gansu 744000,China;School of Agriculture and Bioengineering,Longdong University,Qingyang Gansu 745000,China;Institute of Dryland Agriculture,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China)

机构地区：[1]平凉市农业科学院,甘肃平凉744000 [2]陇东学院农业与生物工程学院,甘肃庆阳745000 [3]甘肃省农业科学院旱地农业研究所,兰州730070

出　　处：《种子》2025年第1期178-187,共10页Seed

基　　金：平凉市科技人才专项计划(PL-STK-2022A-075);平凉市农业科技成果转化项目(平科发[2022]41号);甘肃省技术创新引导计划—津甘双地科技特派员项目(24CXNL009);平凉市重点研发计划(PL-STK-2024A-035);陇东学院博士基金项目(XYBYZK2107);国家自然科学基金(32360455);陇东学院博士基金项目(XYBYZK2205)。

摘　　要：以彩色冬小麦TW10-1154、太黑3号、太蓝1号、张紫麦1号、穗选5号、冬黑10号、农大3153和西农黑等8个品种为试验材料,采用荧光原位杂交(FISH)技术以pSc119.2(绿色)和pTa535(红色)为探针,明确8个彩色冬小麦均属于六倍体材料(AABBDD,2 n=42)。同时,对这8个彩色冬小麦进行常温处理(25℃)和冷胁迫(4℃)处理,并结合NBT组织化学染色法进行ROS定位。结果表明,冷胁迫处理下,张紫麦1号、冬黑10号和西农黑的长势最好,在根尖顶端组织区域ROS信号富集最多,而在伸长组织区域ROS信号随着胚根的生长而逐渐减少,因此,推测ROS信号可能是一种动态传递的信号分子。对冷胁迫下成熟期8个供试材料的根系进行拍摄,结果发现,太蓝1号、张紫麦1号、穗选5号、冬黑10号根须健壮发达,表明这4个品种的小麦生长能力和抗冻性强,适宜陇东地区种植。Using colored winter wheat(Triticum aestivum L.)TW10-1154,Taihei No.3,Tailan No.1,Zhangzimai No.1,Suixuan No.5,Donghei No.10,Nongda 3153 and Xinonghei were selected as experimental materials,fluorescence in situ hybridization(FISH)technique was conducted,using probe pSc119.2(green)and pTa535(red)as probes to confirm all the 8 colored winter wheat as hexaploid materials(AABBDD,2 n=42).At the same time,the 8 samples of colored winter wheat were treated with normal treatment(25℃)and cold stress(4℃),and the ROS localization was performed by NBT histochemical staining.The results showed that under cold stress(4℃),the growth of Zhangzimai No.1,Donghei No.10 and Xinonghei was the best,and the ROS signal was most enriched in the apical tissue region of root tip,while the ROS signal in the extended tissue region was gradually reduced with the growth of radicle.Therefore,it was suggested that ROS signal might be a dynamic signaling molecule.The root system of 8 tested materials at maturity stage under cold stress was photographed.The results showed that the roots of Tailan No.1,Zhangzimai No.1,Suixuan No.5 and Donghei No.10 were robust and developed,indicating that these 4 varieties had strong growth ability and frost resistance,and were suitable for planting in Longdong region.

关 键 词：彩色冬小麦 冷胁迫 活性氧 信号传导 

分 类 号：S512.11[农业科学—作物学]