有机微污染物对反硝化深度脱氮系统的影响研究  

Study on the Effects of Organic Micropollutants on Advanced Denitrification Systems

作　　者：刘淑怡 王德朋 黄开龙 张徐祥[1] LIU Shuyi;WANG Depeng;HUANG Kailong;ZHANG Xuxiang(State Key Laboratory of Pollution Control and Resource Reuse,School of Environment,Nanjing University,Nanjing 210023,China;Nanjing Jiangdao Institute of Environment Research Co.,Ltd.,Nanjing 210019,China)

机构地区：[1]南京大学环境学院污染控制与资源化研究国家重点实验室,江苏南京210023 [2]南京江岛环境科技研究院有限公司,江苏南京210019

出　　处：《能源环境保护》2025年第1期127-134,共8页Energy Environmental Protection

基　　金：江苏省碳达峰碳中和科技创新专项资金资助项目(BE2022861);国家自然科学基金青年基金资助项目(52100068)。

摘　　要：城市污水处理厂二级出水中硝酸盐与有机微污染物(OMPs)常常共存,OMPs对反硝化深度脱氮过程的影响尚不明确。为解析OMPs作用下反硝化脱氮过程响应规律,搭建了反硝化移动床生物膜反应器,并针对OMPs作用下的反硝化性能、OMPs去除效果、微生物胞外聚合物(EPS)响应和电子传递链关键酶活性等方面开展了研究。结果表明:OMPs明显降低反硝化脱氮性能,导致碳、氮去除率均出现波动,且降至70%以下;OMPs中炔雌醇(EE)、雌三醇(E3)、双氯芬酸(DCF)的去除率均在75%以上,而卡马西平(CBZ)去除率维持在20%~44%。在OMPs作用下,微生物首先利用部分EPS作为代谢的有机碳源,随后分泌更多EPS以抵御OMPs的胁迫。同时,电子传递链的关键酶活性受到抑制,使电子传递的有效性降低,进而导致反硝化性能变差。值得关注的是,高浓度的OMPs对系统反硝化效果、EPS含量、电子传递链关键酶活性的冲击更强烈,但微生物的代谢响应比低浓度组更快。该研究为尾水深度脱氮与OMPs的协同去除提供参考。In conventional urban sewage treatment plants,nitrate nitrogen and a wide range of organic micropollutants(OMPs),including endocrine disruptors and pharmaceuticals,frequently coexist in the effluent from secondary treatment processes.These compounds have been demonstrated to exert significant effects on various microorganisms.However,their influence on the advanced denitrification process remains to be comprehensively elucidated.This study aimed to investigate the response of the denitrification process to OMPs through the construction and operation of three denitrification moving bed biofilm reactors.The reactors were employed to evaluate the denitrification removal rate,assess the efficiency of micropollutant removal,analyze the response of extracellular polymeric substances(EPS)in the biofilm,and examine variations in key enzyme activities within the electron transport chain.The findings revealed that the presence of OMPs had a detrimental impact on denitrification efficiency,resulting in instability and a significant reduction in carbon and nitrogen removal rates to below 70%.Concurrently,the removal efficiencies of micropollutants such as ethinyl estradiol(EE),estriol(E3),and diclofenac(DCF)were found to exceed 75%,whereas the removal rates of carbamazepine(CBZ)varied between 20%and 44%.This suggests that different OMPs are handled differently by the denitrification system.During the introduction of micropollutants into the systems,it was observed that microorganisms initially utilized a portion of the EPS as a nutrient source,indicating an important role of EPS in microbial adaptation.Subsequently,in response to the stress induced by OMPs,these microorganisms secreted additional EPS.This response underscores that EPS plays a crucial role in the microbial adaptation to micropollutant stress.Moreover,the activity of key enzymes within the electron transport chain was inhibited due to the presence of micropollutants,thus reducing electron transport efficiency and consequently impairing the overall denitrificat

关 键 词：反硝化脱氮 有机微污染物 深度处理 胞外聚合物 电子传递链 

分 类 号：X703[环境科学与工程—环境工程] X172