毛竹PeACO基因家族鉴定及箨鞘剥除后的表达分析  

Identification of PeACO Gene Family and Expression Analysis After Culm Sheath Removal in Phyllostachys edulis

作　　者：李其旻 兰智鑫 朱唯玮 李翔宇 吴蔼民 林新春[1] LI Qi-Min;LAN Zhi-Xin;ZHU Wei-Wei;LI Xiang-Yu;WU Ai-Min;LIN Xin-Chun(State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,China;College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]浙江农林大学省部共建亚热带森林培育国家重点实验室,杭州311300 [2]华南农业大学林学与风景园林学院,广州510642

出　　处：《农业生物技术学报》2025年第1期55-67,共13页Journal of Agricultural Biotechnology

基　　金：国家重点研发计划(2021YFD2200503-3)。

摘　　要：1-氨基环丙烷-1-羧酸氧化酶(1-aminocyclopropane-1-carboxylate oxidase, ACO)是乙烯合成过程中的关键限速酶之一。本研究以毛竹(Phyllostachys edulis)为材料,对PeACO基因家族进行全基因组鉴定及表达分析,以探究PeACOs在毛竹茎秆木质化进程中的影响。本研究通过与水稻(Oryza sativa) ACO基因序列比对,在毛竹基因组中鉴定得到11条具有完整2OG-FeII_Oxy结构域的PeACOs序列;理化分析显示,PeACOs基因编码304~445个氨基酸,蛋白分子量为34 105.83~49 417.78 D;进化关系和基因结构分析显示,同一分支的基因对在基因结构和蛋白水平上保持高度的保守性;共线性分析得出ACO基因家族在毛竹中共有6对共线性基因,说明基因组复制事件对PeACOs基因数目扩张有重要影响。基因表达分析显示,PeACOs在毛竹各组织均有表达,尤其在快速生长期的笋中表达丰度最高;剥除处于快速生长期的毛竹箨鞘导致毛竹茎秆木质化程度加深,大部分PeACOs基因的表达量显著上调,且具有相似结构的基因对表达趋势相似,提示PeACO基因家族可能在木质化进程中发挥重要作用。本研究为深入探讨毛竹ACO基因家族功能提供了参考依据。1-aminocyclopropane-1-carboxylate oxidase(ACO)is one of the key rate limiting enzymes in the ethylene synthesis process.In this study,genome-wide identification and expression analysis of the PeACO gene family were performed in order to investigate the effects of PeACOs on the lignification process of moso bamboo(Phyllostachys edulis)stalks.In this study,11 sequences of PeACOs with complete 2OG-FeII_Oxy structural domains were identified in the moso bamboo genome by sequence comparison with ACO genes in rice(Oryza sativa);Physicochemical analysis of the proteins showed that these genes encoded 304~445 amino acids,and the molecular weights of the proteins ranged from 34105.83 to 49417.78 D,evolutionary relationship and gene structure analyses showed that pairs of genes in the same branch were highly conserved at the gene structure and protein levels.The collinearity analysis showed that ACO gene family had 6 co-linear gene pairs in moso bamboo,and suggested that genome replication events had an important effect on the expansion of the number of PeACOs genes.PeACOs were expressed in all tissues of moso bamboo,and the highest abundance was especially found in shoots during the rapid growth period.The removal of bamboo clum sheath during the rapid growth period led to the deepening of lignification of the culm.And the expression analysis showed that most PeACOs genes were significantly upregulated by the removal of bamboo sheaths,and indicated that these genes played an important role in this process,and the gene pairs with similar structures have similar expression patterns.This study provides theoretical basis for further study on the function of ACO gene family of bamboo.

关 键 词：毛竹 1-氨基环丙烷-1-羧酸氧化酶(ACO)基因家族 表达分析 木质化 

分 类 号：S795.7[农业科学—林木遗传育种]