基于数据挖掘的腹泻型肠易激综合征动物模型应用分析  

Application Analysis of Animal Models of Diarrhea-predominant Irritable Bowel Syndrome Based on Data Mining

作　　者：罗芳丽[1,2] 孙路强 侯雨君[1] 王司琦 李瑛 周思远[1] LUO Fangli;SUN Luqiang;HOU Yujun;WANG Siqi;LI Ying;ZHOU Siyuan(College of Acupuncture and Massage,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Clinical School of Integrated Traditional and Western Medicine,North Sichuan Medical University,Nanchong 637100,China)

机构地区：[1]成都中医药大学针灸推拿学院,成都611137 [2]川北医学院中西医结合临床医学院,四川南充637100

出　　处：《中国实验方剂学杂志》2025年第2期219-226,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(82074558);成都中医药大学“杏林学者”学科人才科研提升计划项目(QJJJ2022017)。

摘　　要：目的:基于文献数据挖掘探讨国内外腹泻型肠易激综合征(IBS-D)动物模型的造模要素,为完善其造模方法和评价指标提供参考与建议。方法:通过计算机检索中国知网、万方、维普、中华医学期刊全文数据库、PubMed数据库,获取2014—2024年中有关IBS-D动物实验的相关文献,将实验动物种类、性别、体质量、造模方法、造模周期、干预对照方式、成模标准及检测指标进行整理,应用Microsoft Excel 2021软件建立数据库并进行统计,分析IBS-D动物模型的模型特点。结果:归纳总结符合纳入标准的398篇文献,建立IBS-D动物模型多选用SD大鼠、Wistar大鼠和C57BL/6小鼠,动物性别以雄性居多,通常选用6~8周龄的大鼠,小鼠多集中在4~6周龄。干预对照中,西医以匹维溴铵为主,中药以痛泻要方为主,针灸以电针为主。造模方式中,以多因素联合的复合造模方法为主。造模周期多集中在1~14 d和15~30 d。造模成功标准主要根据动物表观情况、粪便情况、内脏敏感性、胃肠动力、行为学、病理等方面进行评价。检测指标主要包括表观指标、病理指标、生化指标、氧化应激、脑肠肽、神经递质、炎症因子、免疫功能、肠道通透性、自噬、凋亡、相关信号通路相关蛋白、肠道菌群及其代谢产物等。结论:IBS-D动物模型目前造模方法较多,尚未形成统一公认的造模方法,相同造模方法在不同研究中的操作及模型评价标准尚不统一。基于数据挖掘的结果,笔者认为多因素联合的复合造模方法更能贴近IBS-D病理生理过程,可以较好地模拟IBS-D患者腹痛、腹泻等复杂的临床症状,具有较高的临床吻合度,是相对比较推荐的造模方法。动物模型各方面均较为符合西医学的标准,中医证候的动物模型相对较少,构建符合中西医临床病证结合要求的IBS-D动物模型未来研究方向之一。Objective:Based on literature data mining,this study explores the modeling elements of diarrhea-predominant irritable bowel syndrome(IBS-D)animal models in China and abroad,providing references and suggestions for improving modeling methods and evaluation indicators.Methods:Relevant literature on IBS-D animal experiments from 2014 to 2024 was retrieved through computer searches in databases such as China National Knowledge Infrastructure(CNKI),Wanfang Data,VIP,Chinese Medical Journals Full-text Database,and PubMed.Information on experimental animal species,gender,body weight,modeling methods,modeling periods,intervention controls,modeling standards,and detection indicators was organized.Microsoft Excel 2021 software was used to establish a database and perform statistical analysis to examine the characteristics of IBS-D animal models.Results:A total of 398 articles that met the inclusion criteria were reviewed.The IBS-D animal models were predominantly established using SD rats,Wistar rats,and C57BL/6 mice.Male animals were more commonly used,with rats typically aged 6-8 weeks and mice aged 4-6 weeks.In terms of interventions,piverium bromide was the main Western medicine,Tongxieyaofang was the primary Chinese medicine,and electroacupuncture was the primary acupuncture method.Among the modeling methods,the multi-factor combined composite modeling approach was the most common.Modeling periods were mainly concentrated between 1-14 days and 15-30 days.The success criteria for modeling were mainly evaluated based on the animal's general condition,fecal appearance,visceral sensitivity,gastrointestinal motility,behavior,and pathology.Detection indicators included apparent indexes,pathological markers,biochemical indicators,oxidative stress,brain-gut peptides,neurotransmitters,inflammatory factors,immune function,intestinal permeability,autophagy,apoptosis,proteins related to relevant signaling pathways,intestinal microbiota and its metabolites,etc.Conclusion:There are various methods for establishing IBS-D animal model

关 键 词：腹泻型肠易激综合征 数据挖掘 动物模型 应用分析 中医证候要素 

分 类 号：R285[医药卫生—中药学] R289[医药卫生—中医学] R287