不同时期朱琏兴奋Ⅰ型针法对糖尿病神经源性膀胱大鼠ROCK、PI3K/AKT通路的影响  

The effect of Zhu Lian′s excitation type Ⅰ acupuncture at different periods on the ROCK and PI3K/AKT pathways in diabetes neurogenic bladder rats

作　　者：林睿 贺煜竣 范郁山[1] 覃宁婧 张卉[1] 黄敬文 LIN Rui;HE Yu-Jun;FAN Yu-Shan(College of Acupuncture and Massage,Guangxi University of Traditional Chinese Medicine,Nanning 530001,Guangxi,China)

机构地区：[1]广西中医药大学针灸推拿学院,广西南宁530001

出　　处：《中国老年学杂志》2025年第1期88-95,共8页Chinese Journal of Gerontology

基　　金：国家自然科学基金(No.82260983);广西研究生教育创新计划项目(No.YCBZ2023149,YCSW2022351)。

摘　　要：目的 观察不同时期朱琏兴奋Ⅰ型针法对糖尿病神经源性膀胱(DNB)大鼠Rho相关卷曲螺旋形成蛋白激酶(ROCK)、磷脂酰激酶3-激酶(PI3K)/蛋白激酶B(AKT)通路的影响,探讨该针法治疗DNB的机制。方法 将50只SD大鼠随机分为对照组、模型组、针法预防组、针法治疗组、针法预防+治疗组各10只。腹腔注射链脲佐菌素制备DNB大鼠模型。朱琏针法选取大鼠“中极”“三阴交”“列缺”“太冲”穴,针法预防组于6~9 w每日捆绑行针法、针法治疗组于10~13 w每日捆绑行针法,针法预防+治疗组于6~13 w每日捆绑行针法,各组无针法干预需要时每日常规捆绑。比较各组膀胱最大容量、残余尿量、膀胱湿重;苏木素-伊红染色观察膀胱组织形态;Western印迹检测PI3K、AKT蛋白表达;酶联免疫吸附试验(ELISA)测定ROCK1、ROCK2、P-PI3K、P-AKT蛋白表达;实时荧光定量-聚合酶链反应(PCR)检测ROCK1、ROCK2、PI3K、AKT mRNA表达。结果 与对照组比较,模型组、针法预防组、针法治疗组、针法预防+治疗组膀胱最大容量、残余尿量、膀胱湿重增加,ROCK蛋白和mRNA表达升高,PI3K/AKT蛋白和mRNA表达降低,差异有统计学意义(P<0.05);与模型组比较,针法治疗组、针法预防组、针法预防+治疗组膀胱最大容量、残余尿量、膀胱湿重降低,ROCK蛋白和mRNA表达降低,PI3K/AKT蛋白和mRNA表达升高,差异有统计学意义(P<0.05);针法治疗组、针法预防组、针法预防+治疗组膀胱最大容量、残余尿量无统计学差异(P>0.05),膀胱湿重依次降低,ROCK蛋白和mRNA表达依次降低,PI3K/AKT蛋白和mRNA表达依次升高,差异有统计学意义(P<0.05)。结论 朱琏兴奋Ⅰ型针法有利于改善大鼠膀胱功能,其机制可能与其下调DNB大鼠膀胱组织中ROCK表达量、上调PI3K/AKT通路表达量有关,而预防性干预的效果要优于发作后干预,预防、治疗联合干预优于单纯预防性干预或发作后干预。Objective To observe the effect of Zhu Lian′s excitation type Ⅰ acupuncture at different periods on Rho associated protein kinase(ROCK) and phosphatidylinositol-3-hydroxykinase(PI3K)/protein kinase B(AKT) pathways in rats with diabetes neurogenic bladder(DNB), and to explore the mechanism of this acupuncture in treating DNB.Methods Fifty SD rats were randomly divided into a control group, a model group, an acupuncture prevention group, an acupuncture treatment group, and an acupuncture prevention+treatment group, with 10 rats in each group. The DNB model of rats was established by intraperitoneal injection of streptozotocin. The "Zhongji" "Sanyinjiao" "Lieque" and "Taichong" acupoints of rats was selected by Zhu Lian′s acupuncture. The acupuncture prevention group was bound with acupuncture every day from 6 to 9 w, the acupuncture treatment group was bound with acupuncture every day from 10 to 13 w, and the acupuncture prevention+treatment group was bound with acupuncture every day from 6 to 13 w. If there was no acupuncture intervention in each group, routine binding was performed every day. The maximum bladder capacity, residual urine volume, and bladder wet weight of each group were compared;bladder tissue morphology was observed by hematoxylin-eosin staining;Western blot was used to detect the expressions of PI3K and AKT protein;enzyme linked immunosorbent assay(ELISA) was used to measure the expressions of ROCK1, ROCK2, P-PI3K, and P-AKT protein;Real time fluorescence quantitative polymerase chain reaction was used to detect the expressions of ROCK1, ROCK2, PI3K, and AKT mRNA.Results Compared with the control group, maximum bladder capacity, residual urine volume, and bladder wet weight were increased, expressions of ROCK protein and mRNA were increased, expressions of PI3K/AKT protein and mRNA were decreased in the model group, acupuncture prevention group, acupuncture treatment group, and acupuncture prevention+treatment group, the difference was statistically significant(P<0.05). Compared with the m

关 键 词：朱琏兴奋Ⅰ型针法 治未病 糖尿病神经源性膀胱 Rho相关卷曲螺旋形成蛋白激酶(ROCK) 磷脂酰激酶3-激酶(PI3K)/蛋白激酶B(AKT)通路 

分 类 号：R245.3[医药卫生—针灸推拿学]