α-常春藤皂苷与奥沙利铂联用对HCT116结肠癌细胞的化疗增敏作用  

Chemosensitization of HCT116 colon cancer cells by α-hederin in combination with oxaliplatin

作　　者：周婷婷 朱文坚 冯慧 程海波[1,2,3] 沈卫星 ZHOU Ting-Ting;ZHU Wen-Jian;FENG Hui(The First Clinical College of Nanjing University of Chinese Medicine,Nanjing 210023,Jiangsu,China)

机构地区：[1]南京中医药大学第一临床医学院,江苏南京210023 [2]江苏省中医药防治肿瘤协同创新中心 [3]南京中医药大学附属医院肿瘤内科

出　　处：《中国老年学杂志》2025年第1期95-101,共7页Chinese Journal of Gerontology

基　　金：国家自然科学基金面上项目(81973523);江苏省研究生科研与实践创新计划项目(KYCX22_1895)。

摘　　要：目的 探讨α-常春藤皂苷(HED)与奥沙利铂(OXA)联用对HCT116结肠癌(CRC)细胞的化疗增敏作用。方法 噻唑蓝(MTT)法检测α-HED与OXA单用或联用对HCT116细胞增殖抑制率的影响;CompuSyn软件检测协同效果,得到最佳给药剂量;采用平板克隆实验、流式细胞术及Western印迹法检测α-HED与OXA联用对HCT116细胞克隆及凋亡的影响。体内实验构建HCT116细胞裸鼠皮下移植瘤模型,分为Control、α-HED、L-OXA、N-OXA及α-HED+L-OXA组,观察α-HED与OXA单用或联用处理CRC模型对肿瘤生长的影响。结果 6μmol/L α-HED显著促进25μmol/L OXA对HCT116细胞的增殖抑制率和克隆形成率,显著促进细胞凋亡(P<0.05)。α-HED与OXA单用显著抑制CRC裸鼠移植瘤的生长增殖,联用时抑瘤效果更明显(P<0.05)。α-HED与OXA联用显著下调Ki67、CD31的表达,较α-HED组和OXA低剂量组更显著(P<0.05)。结论 α-HED与OXA联用在体内外存在对HCT116 CRC细胞的化疗增敏效果。Objective To investigate the chemosensitizing effect of combining α-hederin(HED) with oxaliplatin(OXA) on HCT116 colorectal cancer(CRC) cells.Methods The MTT assay was used to detect the inhibitory effects of α-HED and OXA, both individually and in combination, on the proliferation of HCT116 cells. Synergistic interactions and optimal dosing regimens were analyzed using CompuSyn software. Plate colony formation assays, flow cytometry, and Western blot analysis were conducted to evaluate the combined effects of α-HED and OXA on colony formation and apoptosis in HCT116 cells. For in vivo experiments, a subcutaneous xenograft model was established by implanting HCT116 cells into nude mice, which were divided into Control, α-HED, L-OXA, N-OXA, and α-HED+L-OXA groups. The effects of α-HED and OXA, individually and in combination, on tumor growth in the CRC model were subsequently observed.Results The inhibitory effects of 25 μmol/L OXA on both the proliferation and colony formation rate of HCT116 cells were significantly enhanced by 6 μmol/L α-HED, apoptosis was significantly promoted by 6 μmol/L α-HED(P<0.05). Both α-HED and OXA alone effectively inhibited the growth and proliferation of CRC xenografts in nude mice, the antitumor effect was significantly more pronounced when the two agents were administered in combination(P<0.05).α-HED and OXA were administered in combination which were significantly downregulated the expressions of Ki67 and CD31, with a more significant reduction observed in comparison to the α-HED and low-dose OXA groups(P<0.05).Conclusions The combination of α-HED and OXA could exhibite chemosensitization effects on HCT116 CRC cells both in vitro and in vivo.

关 键 词：α-常春藤皂苷 奥沙利铂 结肠癌 HCT116细胞 化疗增敏 

分 类 号：R285.5[医药卫生—中药学]