工业丝状真菌生物制造的现状和展望  

Current status and prospect of industrial filamentous fungi in biomanufacturing

作　　者：杜志强 门萍 周宇 张璇 黄雪年 吕雪峰[1,2,3] DU Zhiqiang;MEN Ping;ZHOU Yu;ZHANG Xuan;HUANG Xuenian;LYU Xuefeng(Shandong Provincial Key Laboratory of Synthetic Biology,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,China;Qingdao New Energy Shandong Laboratory,Qingdao 266101,China;Shandong Energy Institute,Qingdao 266101,China;Institute for Smart Materials&Engineering,University of Jinan,Jinan 250022,China)

机构地区：[1]中国科学院青岛生物能源与过程研究所、山东省合成生物学重点实验室,青岛266101 [2]青岛新能源山东省实验室,青岛266101 [3]山东能源研究院,青岛266101 [4]济南大学智能材料与工程研究院,济南250022

出　　处：《中国科学院院刊》2025年第1期36-46,共11页Bulletin of Chinese Academy of Sciences

基　　金：国家自然科学基金重点项目(22338012);国家自然科学基金面上项目(32170098);山东省重大科技创新工程(2023CXGC010603);泰山学者攀登计划专家(NO.tspd20240817);泰山学者青年专家(NO.tsqn202103130)。

摘　　要：丝状真菌作为支撑生物制造产业发展的一类重要工业微生物,广泛应用于医药、食品、农业和材料化工等多个领域,与人类日常生活密切相关。近年来,合成生物技术的发展进一步推动了工业丝状真菌在生物制造领域的应用。文章综述了该领域的最新进展,涵盖了工业丝状真菌遗传改造技术的最新发展,通过理性改造策略推动现有产品生产工艺提质增效的成功案例,以及工业菌株作为底盘细胞在生产植物源天然产物、生物基化学品和功能性蛋白方面的新技术。文章对上述工作进行了总结和分析,并对工业丝状真菌在生物制造领域的发展前景和面临的挑战进行了展望和讨论。Filamentous fungi,a critical class of industrial microorganisms pivotal to the biomanufacturing industry,has extensive applications in various fields such as pharmaceuticals,food,agriculture,and chemical materials,which are playing a vital role in human life.Recently,innovations in synthetic biology have further driven the application of industrial filamentous fungi in biomanufacturing,highlighting their critical role in this field.This article presents a comprehensive review of the recent progress in the field,covering the latest developments in genetic modification techniques for industrial filamentous fungi,successful cases of rational strain improvement strategies aimed to enhance the quality and efficiency of current product manufacturing processes,and innovative technologies that utilize industrial strains as chassis cells for the production of plant natural products,bio-based chemicals,and functional proteins.The review summarizes and analyzes the work that has been discussed,and explores the prospects and challenges for industrial filamentous fungi in biomanufacturing.

关 键 词：合成生物学 生物制造 工业丝状真菌 细胞工厂 生物基化学品 

分 类 号：Q939.97[生物学—微生物学]