血清MMP-9、CHL1、IL-13在支气管哮喘合并肺炎支原体感染患儿中的水平变化及检测价值  

Changes in Serum MMP-9,CHL1 and IL-13 Levels and Their Diagnostic Value in Children with Bronchial Asthma Complicated with Mycoplasma Pneumoniae Infection

作　　者：高伟霞 李德亮[1] 张靖[1] 刘阳[1] 吕亚洲[1] 侯海燕[1] GAO Weixia;LI Deliang;ZHANG Jing;LIU Yang;L Yazhou;HOU Haiyan(Department of Pediatrics,Nanyang Central Hospital,Nanyang 473000,China)

机构地区：[1]南阳市中心医院儿科,南阳473000

出　　处：《福建医科大学学报》2024年第6期407-412,共6页Journal of Fujian Medical University

基　　金：河南省医学科技攻关计划(联合共建)项目(LHGJ20191453)。

摘　　要：目的探讨血清基质金属蛋白酶-9(MMP-9)、神经细胞黏附分子L1样蛋白(CHL1)、白细胞介素-13(IL-13)在支气管哮喘合并肺炎支原体(MP)感染患儿中的水平变化及检测价值。方法选取2020年3月-2023年12月支气管哮喘患儿83例,根据是否合并MP感染分为感染组(23例)和无感染组(60例),比较2组患儿的基线资料[性别、年龄、体质量、支气管哮喘病程气道重塑指标气道壁厚度/气道管腔外径(T/D)、气道壁面积占气道总截面积百分比(WA%)、肺功能指标第1秒用力呼气量/1秒用力呼气量预测值的百分比(FEV 1%)、用力肺活量(FVC)、最大呼气50%流量(MEF50)]、血清MMP-9、CHL1、IL-13水平,分析血清MMP-9、CHL1、IL-13水平与气道重塑、肺功能的关系,采用受试者工作特征(ROC)曲线获取各血清指标在支气管哮喘合并MP感染中的最佳截断值,并以最佳截断值为界将各血清指标分为低表达与高表达,采用相对危险度(RR)分析血清MMP-9、CHL1、IL-13对支气管哮喘合并MP感染风险的影响。结果感染组T/D、WA%高于无感染组,FEV 1%、FVC、MEF50低于无感染组(P<0.05);感染组血清MMP-9、CHL1、IL-13水平高于无感染组(P<0.001),血清MMP-9、CHL1、IL-13与T/D、WA%呈正相关,与FEV 1%、FVC、MEF50呈负相关(P<0.001)。ROC曲线分析显示,血清MMP-9、CHL1、IL-13在支气管哮喘合并MP感染中的最佳截断值分别为138.21μg/L、8.62 ng/mL、10.18 ng/L;血清MMP-9、CHL1、IL-13高表达的支气管哮喘患儿合并MP感染的风险分别是低表达患儿的5.738、6.202、4.937倍(P<0.05)。结论血清MMP-9、CHL1、IL-13在支气管哮喘合并MP感染患儿中呈异常升高表达,与气道重塑、肺功能显著相关,可为临床诊治支气管哮喘合并MP感染提供有效信息。Objective To investigate the levels and diagnostic value of serum matrix metalloproteinase-9(MMP-9),nerve cell adhesion molecule L1-like protein(CHL1)and interleukin-13(IL-13)in children with bronchial asthma complicated with mycoplasma pneumonitis(MP)infection.Methods A total of 83 children with bronchial asthma from our hospital from March 2020 to December 2023 were selected and divided into an infection group(23 cases)and a non-infection group(60 cases)based on whether they were infected with MP.The baseline data of the two groups were compared,including gender,age,body weight,airway remodeling indicators[airway wall thickness/airway lumen outer diameter(T/D),airway wall area percentage of total airway cross-sectional area(WA%)],lung function indicators[percentage of forced expiratory volume in one second/predicted value of forced expiratory volume in one second(FEV 1%),forced vital capacity(FVC),maximum expiratory flow rate at 50%(MEF50)],serum levels of MMP-9,CHL1,and IL-13.The relationship between serum levels of MMP-9,CHL1,and IL-13 and airway remodeling and lung function was analyzed.The receiver operating characteristic(ROC)curve was used to obtain the optimal cutoff value for each serum indicator in bronchial asthma with MP infection,and the optimal cutoff value was used to divide each serum indicator into low expression and high expression.The relative risk(RR)analysis was used to analyze the impact of serum levels of MMP-9,CHL1,and IL-13 on the risk of bronchial asthma with MP infection.Results The T/D and WA%in the infection group were higher than those in the non-infection group,while the FEV 1%,FVC,and MEF50 were lower than those in the non-infection group(P<0.05).The levels of serum MMP-9,CHL1,and IL-13 in the infection group were higher than those in the non-infection group(P<0.001).Serum MMP-9,CHL1,and IL-13 were positively correlated with T/D and WA%,and negatively correlated with FEV 1%,FVC,and MEF50(P<0.001).ROC curve analysis showed that the optimal cutoff values for serum MMP-9,CHL1,and IL-

关 键 词：支气管哮喘 肺炎支原体感染 基质金属蛋白酶-9 神经细胞黏附分子L1样蛋白 白细胞介素-13 检测价值 

分 类 号：R56[医药卫生—呼吸系统]