藏药十五味龙胆花丸对哮喘模型幼鼠肺泡灌洗液中嗜酸性粒细胞和炎症介质表达水平的影响  

Effects of Tibetan medicine Shiwuwei Longdanhua pill on the eosinophils and expression levels of inflammatory mediators in alveolar lavage fluid of asthmatic model young rats

作　　者：葛利吉 翟晓婷 杨旭东 杨昆 李晶 李旭 冀旭 张红燕 GE Liji;ZHAI Xiaoting;YANG Xudong;YANG Kun;LI Jing;LI Xu;JI Xu;ZHANG Hongyan(Affiliated Hospital of Xizang Minzu University,Xianyang 712082;School of Basic Medicine,Xi'an Jiaotong University Health Science Center,Xi'an 710061;School of Basic Medicine,Xizang Minzu University,Xianyang 712082,China)

机构地区：[1]西藏民族大学附属医院,陕西咸阳712082 [2]西安交通大学医学部基础医学院,陕西西安710061 [3]西藏民族大学基础医学院,陕西咸阳712082

出　　处：《临床医学研究与实践》2025年第4期20-23,共4页Clinical Research and Practice

基　　金：2020年度西藏自治区自然科学基金立项项目(No.XZ202001ZR0091G)。

摘　　要：目的探究藏药十五味龙胆花丸对哮喘模型幼鼠肺泡灌洗液中嗜酸性粒细胞和炎症介质表达水平的影响。方法将50只SD雌性BABL/c幼鼠随机分为对照组、哮喘组、布地奈德组、十五味龙胆花丸组、布地奈德+十五味龙胆花丸组,每组10只。除对照组外,其余4组均通过注射卵清蛋白(OVA)与氢氧化铝粉末制备的生理盐水混合液进行致敏处理,建立哮喘幼鼠模型。建模后对照组和哮喘组用等体积磷酸盐缓冲液(PBS)灌胃,布地奈德组、十五味龙胆花丸组和布地奈德+十五味龙胆花丸组分别用0.26 mg/kg布地奈德混悬液、200μL十五味龙胆花丸混悬液和0.26 mg/kg布地奈德混悬液+200μL十五味龙胆花丸混悬液灌胃,持续给药10 d。比较各组幼鼠肺肺泡灌洗液中嗜酸性粒细胞比例、白细胞介素-5(IL-5)、肿瘤坏死因子-α(TNF-α)和白细胞介素-33(IL-33)相对表达量。结果哮喘组肺泡灌洗液中嗜酸性粒细胞比例、IL-5、IL-33相对表达量大于对照组(P<0.05);布地奈德组、十五味龙胆花丸组和布地奈德+十五味龙胆花丸组肺泡灌洗液中嗜酸性粒细胞比例、IL-5、IL-33相对表达量低于哮喘组(P<0.05);十五味龙胆花丸组和布地奈德+十五味龙胆花丸组肺泡灌洗液中IL-5相对表达量低于布地奈德组(P<0.05)。哮喘组和布地奈德组肺泡灌洗液中TNF-α相对表达量大于对照组(P<0.05);十五味龙胆花丸组和布地奈德+十五味龙胆花丸组肺泡灌洗液中TNF-α相对表达量低于哮喘组(P<0.05);十五味龙胆花丸组和布地奈德+十五味龙胆花丸组肺泡灌洗液中TNF-α相对表达量低于布地奈德组(P<0.05)。结论藏药十五味龙胆花丸能有效降低哮喘幼鼠肺泡灌洗液中嗜酸性粒细胞比例,抑制局部IL-5、TNF-α、IL-33释放,减轻气道炎症反应,有望用于儿童哮喘治疗中。Objective To explore the effects of Tibetan medicine Shiwuwei Longdanhua pill on the eosinophils and expression of inflammatory mediators in alveolar lavage fluid of asthmatic model young rats.Methods Fifty SD female BABL/c young rats were randomly divided into control group,asthma group,budesonide group,Shiwuwei Longdanhua pill group,budesonide+Shiwuwei Longdanhua pill group,with 10 rats in each group.In addition to the control group,the other four groups were sensitized by injection of ovalbumin(OVA)and normal saline mixture prepared by aluminum hydroxide powder to establish a rat model of asthma.After modeling,the control group and the asthma group were intragastrically administered with an equal volume of phosphate buffered saline(PBS).The budesonide group,the Shiwuwei Longdanhua pill group,and the budesonide+Shiwuwei Longdanhua pill group were intragastrically administered with 0.26 mg/kg budesonide suspension,200μL Shiwuwei Longdanhua pill suspension,and 0.26 mg/kg budesonide suspension+200μL Shiwuwei Longdanhua pill suspension,respectively,for 10 d.The proportion of eosinophils,the relative expression of interleukin-5(IL-5),tumor necrosis factor-α(TNF-α)and interleukin-33(IL-33)in bronchoalveolar lavage fluid of young rats in each group were compared.Results The proportion of eosinophils and the relative expression of IL-5 and IL-33 in bronchoalveolar lavage fluid of the asthma group were higher than those of the control group(P<0.05);the proportion of eosinophils and the relative expression of IL-5 and IL-33 in bronchoalveolar lavage fluid of the budesonide group,the Shiwuwei Longdanhua pill group and the budesonide+Shiwuwei Longdanhua pill group were lower than those of the asthma group(P<0.05);the relative expression of IL-5 in alveolar lavage fluid of the Shiwuwei Longdanhua pill group and the budesonide+Shiwuwei Longdanhua pill group was significantly lower than that of the budesonide group(P<0.05).The relative expression of TNF-αin bronchoalveolar lavage fluid in the asthma group and the budeson

关 键 词：哮喘 十五味龙胆花丸 嗜酸性粒细胞 辅助性T淋巴细胞 炎症反应 

分 类 号：R275.9[医药卫生—中西医结合]