基于普鲁士蓝离子缔合物反应共振散射猝灭法检测谷胱甘肽  

Glutathione was determined by resonance scattering quenching method based on Prussian blue ion association reaction

作　　者：左卫元 陈盛余 林继锦 欧阳辉祥 ZUO Wei-Yuan;CHEN Sheng-Yu;LIN Ji-Jin;OUYANG Hui-Xiang(College of Chemical and Environmental Engineering,Baise University,Baise 533000,China;Laboratory of Eco-Environment Analysis and Pollution Control in Western Guangxi,Baise 533000,China)

机构地区：[1]百色学院化学与环境工程学院,百色533000 [2]桂西区域生态环境分析和污染控制实验室,百色533000

出　　处：《实验室检测》2024年第8期29-32,共4页Laboratory Testing

摘　　要：目的 通过共振散射光谱法探索共振散射光谱猝灭法测定痕量谷胱甘肽,以提高方法的灵敏度。方法 在pH为3.0的磷酸-硼酸-醋酸缓冲溶液(BR缓冲溶)中,Fe(Ⅲ)与K_(4)[Fe(CN)_(6)]反应生成普鲁士蓝离子缔合物,测得体系在337 nm处有较强共振散射效应,当加入谷胱甘肽后,谷胱甘肽分子中的巯基可还原Fe(Ⅲ)为Fe(Ⅱ),使体系发生猝灭,导致体系共振散射猝灭效应减弱。结果 谷胱甘在浓度0.010~2.0μg/mL范围内,线性回归方程为ΔI=299.77ρ+774.52,r^(2)为0.9929,检出限为0.0024μg/mL。结论 该法操作简便,灵敏度高,用于药物中的谷胱甘肽的测定,得到满意的结果。Objective To explore the determination of trace glutathione by resonance scattering spectral quenching method and improve the sensitivity of the method.Methods In the pH 3.0 phosphoric acid-boric acid-acetic acid buffer solution(BR buffer),Fe(Ⅲ)reacts with K4[Fe(CN)6]to form Prussian blue ion associates,and the system exhibits a strong resonance scattering effect at 337 nm.When glutathione is added,the sulfhydryl group in glutathione molecules can reduce Fe(Ⅲ)to Fe(Ⅱ),causing quenching of the system and weakening the resonance scattering quenching effect.Results The linear regression equation for glutathione within the concentration range of 0.010 to 2.0μg/mL isΔI=299.77ρ+774.52,with an r^(2) of 0.9929 and a detection limit of 0.0024μg/mL.Conclusion This method is simple and highly sensitive,and satisfactory results are obtained when used for the determination of glutathione in drugs.

关 键 词：谷胱甘肽 共振散射猝灭法 普鲁士蓝离子缔合物 

分 类 号：O657.3[理学—分析化学] TQ460.72[理学—化学]