高效液相色谱法同时测定桂枝肌瘤丸中3种成分含量  

Simultaneous determination of three components in Guizhi Jiliu Pills by high performance liquid chromatography

作　　者：曹薇薇 强润东 张春雨 薄会娜 王志平 石可歆 姜颖 CAO Wei-Wei;QIANG Run-Dong;ZHANG Chun-Yu;BO Hui-Na;WANG Zhi-Ping;SHI Ke-Xin;JIANG Ying(Tangshan Food and Drug Comprehensive Inspection and Testing Center,Tangshan 063000,China)

机构地区：[1]唐山市食品药品综合检验检测中心,唐山063000

出　　处：《实验室检测》2024年第8期25-28,共4页Laboratory Testing

基　　金：河北省药品监督管理局科技计划资助(2023ZC1023)

摘　　要：目的 建立同时测定桂枝肌瘤丸中芍药苷、桂皮醛及丹皮酚3种成分含量的方法。方法 采用反相高效液相色谱(Reversed Phase High Performance Liquid Chromatography,RP-HPLC)波长切换法,色谱柱ES Epic C_(18)(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸水溶液梯度洗脱,流速1.0 mL/min,柱温25℃,检测波长为230 nm(0~25 min)、290 nm(25~41 min)、274 nm(41~51 min)。结果 芍药苷、桂皮醛、丹皮酚线性范围分别为3.6169~90.4232μg/m L(r=0.9993)、1.8388~45.9695μg/m L(r=0.9999)、2.0240~50.5994μg/m L(r=0.9999)。平均加样回收率分别为95.4%(RSD=1.2%)、93.0%(RSD=0.7%)和94.2%(RSD=1.2%)。结论 本方法准确、灵敏,专属性强,适用于桂枝肌瘤丸的质量控制。Objective To establish a method for determining the contents of paeoniflorin,cinnamaldehyde and paeonol in Guizhi Jiliu Pills.Methods The RP-HPLC wavelength switching method was used,the analysis was achieved on an ES Epic C_(18)(4.6 mm×250 mm,5μm)column,the mobile phase was acetonitrile-0.1%aqueous solution of phosphoric acid gradient elution,the flow rate was 1.0 mL/min,the column temperature was 25℃and the detection wavelength were 230 nm(0~25 min),290 nm(25~41 min)and 274 nm(41~51 min).Results The linear ranges of paeoniflorin,cinnamaldehyde and paeonol were 3.6169~90.4232μg/mL(r=0.9993),1.8388~45.9695μg/mL(r=0.9999),2.0240~50.5994μg/mL(r=0.9999).The average sample recovery rates were 95.4%(RSD=1.2%),93.0%(RSD=0.7%)and 94.2%(RSD=1.2%)respectively.Conclusion This method is accurate,sensitive,and has strong specificity.It can be used for the quality control of Guizhi Jiliu Pills.

关 键 词：桂枝肌瘤丸 RP-HPLC波长切换法 含量测定 芍药苷 桂皮醛 丹皮酚 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]