秦艽中栎瘿酸提取工艺优化和含量测定方法建立  

Optimization of extraction process and establishment of content determination method for roburic acid in Gentiana macrophylla

作　　者：丁金有 余红 龚平双 DING Jin-You;YU Hong;GONG Ping-Shuang(Shangrao Inspection and Testing Certification Institute,Shangrao 334000,China)

机构地区：[1]上饶市检验检测认证院,上饶334000

出　　处：《实验室检测》2024年第8期21-24,共4页Laboratory Testing

基　　金：江西省药品监督管理局科研项目(2022JS21)

摘　　要：目的 通过建立秦艽中栎瘿酸提取工艺和含量测定方法,优选秦艽的炮制工艺。方法 本研究采用超声辅助法提取秦艽中的栎瘿酸,并通过HPLC-ELSD的方法测定4种炮制秦艽饮片种栎瘿酸的含量。结果 超声提取秦艽中栎瘿酸的最佳工艺条件为:乙醇体积分数75%、超声时间为45 min、料液比为1∶10、超声温度为30℃时,栎瘿酸的得率最高为3.40 mg/g。结论 建立的栎瘿酸提取工艺和含量测定方法重复性好、稳定可靠,可为优选秦艽饮片的炮制工艺提供参考。Objective To establish an extraction process and content determination method for quercetin in Gentiana macrophylla,and optimize the processing technology of Gentiana macrophylla.Methods This study used ultrasound assisted extraction to extract quercetin from Gentiana macrophylla,and determined the content of quercetin in four processed Gentiana macrophylla decoction pieces by HPLC-ELSD.Results The optimal process conditions for ultrasonic extraction of quercetin from Gentiana macrophylla are:ethanol volume fraction of 75%,ultrasonic time of 45 minutes,solid-liquid ratio of 1∶10,and ultrasonic temperature of 30℃.The highest yield of quercetin is 3.40 mg/g.Conclusion The established extraction process and content determination method for quercetin galls have good repeatability,stability,and reliability,which can provide reference for optimizing the processing technology of Gentiana macrophylla decoction pieces.

关 键 词：秦艽 栎瘿酸 超声提取法 正交试验 含量测定 

分 类 号：R284.1[医药卫生—中药学]