冷消解测定血清碘方法与国标法的比较分析  

Comparative analysis of serum iodine determination by cold digestion method and national standard method

作　　者：陈贵莲 丁黎 张颖 李春春 王晓 CHEN Gui-Lian;DING Li;ZHANG Ying;LI Chun-Chun;WANG Xiao(Meinian Health Industry(Group)Co.,Ltd.,Changsha Company,Changsha 410000,China;Changsha Silk-Road Medical Technology Co.,Ltd.,Changsha 410000,China)

机构地区：[1]美年大健康产业(集团)有限公司长沙公司,长沙410000 [2]长沙塞克陆德医疗科技有限公司,长沙410000

出　　处：《实验室检测》2024年第12期121-124,共4页Laboratory Testing

摘　　要：目的 评价冷消解测定血清碘方法在临床应用中的优势和价值。方法 应用长沙塞克陆德的DAT–500型碘元素综合检测仪和冷消解血清碘检测试剂(专利:CN113340888A用于血清定量检测的试剂、试剂盒及检测方法)与国标《血清中碘的测定砷铈催化分光光度法》方法学适用性实验对比。结果 0~300μg/L碘含量区间,5批次标准曲线相关系数绝对值超0.9995,检出限2.1μg/L;9份不同浓度血清样加标回收率94.6%~108.8%;3种浓度血清样各测6批次,RSD为3.41%~4.01%,样品加标回收率94.6%~108.8%;20例血清样本测定值与国标法差异无统计学意义(t=-1.660,P=0.113>0.05)。结论 冷消解法检测血清碘精度与准度高,操作简便耗时短、温控要求低,试剂常温易存运,应用前景佳。Objective To evaluate the advantage clinical value of cold digestion method and national standard method in the determination of serum iodine.Methods The DAT-500 iodine detector of Changsha Seclude and the cold digested serum iodine detection reagent(patent:CN113340888A reagent kit and detection method for serum quantitative detection)were used to compare with the national standard Determination of Iodine in Serum by Catalytic Spectrophotometry.Results From 0 to 300μg/L iodine content range,the absolute value of correlation coefficient of standard curve of 5 batches exceeded 0.9995,the detection limit was 2.1μg/L;the recovery rate of 9 serum samples with different concentrations was 94.6%~108.8%;6 batches of 3 serum samples with different concentrations were tested,the RSD was 3.41%~4.01%,and the recovery rate was 94.6%~108.8%;there was no statistical significance between 20 serum samples and national standard method(t=-1.660,P=0.113>0.05).Conclusion Cold digestion method has the advantages of high accuracy and accuracy,simple operation,short time consuming,low demand for temperature control,easy storage and transportation at room temperature.

关 键 词：冷消解血清碘检测试剂 血清碘自动检测仪 血清碘检测 

分 类 号：R446.11[医药卫生—诊断学]