化学发光免疫法测定血清甲状腺功能三项  

Serum thyroid function was measured by chemiluminescence immunoassay

作　　者：许新 XU Xin(Tianjin Medical University General Hospital,Tianjin 300050,China)

机构地区：[1]天津医科大学总医院,天津300050

出　　处：《实验室检测》2024年第12期117-120,共4页Laboratory Testing

摘　　要：目的 建立化学发光免疫(Chemiluminescence immunoassay,CLIA)法检测血清甲状腺功能三项。方法 回顾性分析天津医科大学总医院2023年2月—2024年5月期间收治的甲状腺功能亢进者共121例,甲状腺功能减退共101例,甲状腺功能正常患者共67例的临床资料,研究对象均使用CLIA法检查血清促甲状腺素(TSH)、游离甲状腺素(FT4)、游离三碘甲状腺原氨酸(FT3),随后统计CLIA系统监测TSH、FT4、FT3的线性范围、精密度、准确度。结果 TSH、FT4、FT3线性相关系数(r)≥0.990,斜率(b)在0.95~1.00内。TSH、FT4、FT3的批内、批间精密度分别小于1/4TEa(CLIA’88)、1/3TEa(CLIA’88)。TSH、FT4、FT3偏倚均小于1/2TEa(CLIA’88)。CLIA法检测的甲状腺功能亢进者、甲状腺功能减退、甲状腺功能正常者TSH、FT4、FT3水平均高于RAI法检测所得的TSH、FT4、FT3水平(P<0.05)。结论 CLIA法对血清甲状腺功能三项检测的准确度、精密度良好,为临床检测血清甲状腺功能三项提供重要依据。Objective To establish a chemiluminescence immunoassay(CLIA)for the detection of serum thyroid function.Methods The clinical data of 121 patients with hyperthyroidism,101 patients with hypothyroidism and 67 patients with normal thyroid function admitted to Tianjin Medical University General Hospital were retrospectively analyzed from February 2023 to May 2024.The levels of serum thyroid stimulating hormone(TSH),free thyroxine(FT4)and free triiodothyronine(FT3)were examined by CLIA,and then the linear range,precision and accuracy of TSH,FT4 and FT3 monitored by CLIA system were counted.Results The linear correlation coefficient(r)values of TSH,FT4 and FT3 were≥0.990,and slope(b)was within 0.95-1.00.The intra-batch and inter-batch precision values of TSH,FT4 and FT3 were less than 1/4TEa(CLIA’88)and 1/3TEa(CLIA’88)respectively.The bias of TSH,FT4 and FT3 were less than 1/2TEa(CLIA’88).The levels of TSH,FT4 and FT3 in patients with hyperthyroidism,hypothyroidism and normal thyroid function detected by CLIA were higher than those detected by RAI(P<0.05).Conclusion CLIA has good accuracy and precision in the detection of three items of serum thyroid function,and provides an important basis for clinical detection of three indexes of serum thyroid function.

关 键 词：化学发光免疫法 促甲状腺素 游离甲状腺素 游离三碘甲状腺原氨酸 

分 类 号：R446.6[医药卫生—诊断学]