羟苯磺酸钙对比浊法尿总蛋白检测试剂的干扰与抗干扰研究  

Study on the interference and anti interference of calcium dobesilate in turbidity urine total protein detection reagent

作　　者：郝雪 赵海洋 夏雨弘 程超 HAO Xue;ZHAO Hai-Yang;XIA Yu-Hong;CHENG Chao(Dirui Medical Technology Co.,Ltd.,Changchun 132000,China)

机构地区：[1]迪瑞医疗科技股份有限公司,长春132000

出　　处：《实验室检测》2024年第12期113-116,共4页Laboratory Testing

摘　　要：目的 探讨羟苯磺酸钙对迪瑞和罗氏尿总蛋白测定试剂盒(比浊法)的干扰及抗干扰方法。方法 (1)按照国家标准WS/T 416—2013《干扰实验指南》,采用低、中、高3个浓度的尿液基质,分别与羟苯磺酸钙配制成不同浓度梯度的羟苯磺酸钙干扰样本,使用迪瑞和罗氏2个厂家的尿总蛋白测定试剂盒(比浊法)检测尿总蛋白浓度,计算干扰样本与基础对照样本检测结果的相对偏差。(2)使用不同测定波长分别测定低、中、高三个浓度的尿液基质配制的0 mmol/L与1 mmol/L羟苯磺酸钙干扰样本,使用迪瑞和罗氏2个厂家的尿总蛋白测定试剂盒(比浊法)测定尿总蛋白浓度,计算与505 nm测定波长下对照样本的相对偏差。结果 (1)当羟苯磺酸钙浓度为0.4 mmol/L时,低、中浓度尿液基质的检测结果偏差开始明显超出干扰可接受范围,且随着羟苯磺酸钙浓度的增加,尿总蛋白检测结果的受干扰程度亦相应增强。在同一药物浓度下,尿总蛋白浓度越低,干扰越明显。(2)当测试波长为800 nm时,可显著降低羟苯磺酸钙对迪瑞和罗氏比浊法检测尿总蛋白试剂的干扰,且干扰偏差均在干扰可接受范围内。结论 羟苯磺酸钙对尿总蛋白测定试剂盒(比浊法)会产生不同程度的负干扰,应引起重视。Objective To investigate the interference of calcium dobesilate on the urine total protein determination test kits(turbidimetric method)and the anti-interference method.Methods(1)According to the national standard WS/T 416-2013 Interference Test Guide,low,medium,and high concentrations of urine matrix were used to prepare calcium dobesilate interference samples of different concentration gradients,and the urine total protein concentration was detected using the urine total protein determination test kits(turbidimetric method)of Dirui and Roche two factories.The relative deviation between the interference sample and the reference control sample was calculated.(2)The urine matrix was prepared at low,medium,and high concentrations,and the 0 mmol/L and 1 mmol/L calcium dobesilate interference samples were prepared using different wavelengths.The urine total protein concentration was determined using the urine total protein determination test kits(turbidimetric method)of Dirui and Roche two factories,and the relative deviation with respect to the 505 nm measurement wavelength was calculated.Results(1)When the calcium dobesilate concentration was 0.4 mmol/L,the deviation of the urine total protein detection results in the low and medium concentration urine matrix began to deviate significantly from the acceptable interference range,and the degree of interference on urine total protein detection increased as the calcium dobesilate concentration increased.At the same drug concentration,the greater the urine total protein concentration was,the more obvious the interference was.(2)When the test wavelength was 800 nm,the interference of calcium dobesilate on the Dirui and Roche turbidimetric method urine total protein test kits could be significantly reduced,and the deviation of interference was within the acceptable interference range.Conclusion Calcium dobesilate can produce varying degrees of negative interference on the total protein determination assay(turbidimetry method),which should be taken seriously.

关 键 词：羟苯磺酸钙 尿总蛋白 比浊法 干扰 

分 类 号：R446.12[医药卫生—诊断学]