脂浊对免疫透射比浊法检测前白蛋白的干扰效果研究  

Study on interference effect of lipid turbidity on the detection of prealbumin by immune nephelometry

作　　者：李芹 LI Qin(Fengxian District Institute of Dermatology and Prevention,Shanghai 201408,China)

机构地区：[1]上海市奉贤区皮肤病防治所,上海201408

出　　处：《实验室检测》2024年第12期109-112,共4页Laboratory Testing

摘　　要：目的 探讨脂浊对免疫透射比浊法检测前白蛋白的干扰效果。方法 选取上海市奉贤区皮肤病防治所2021年2月—2024年1月期间40份来自黄瘤病患者的无脂浊血清样本,另选取40份黄瘤病患者的脂浊血清样本。采取免疫透射比浊法对80份血清样本进行前白蛋白水平检测,随后对40份无脂浊血清样本进行方法重复性分析,对40份脂浊血清样本进行脂浊干扰效果分析。结果 前白蛋白批内精密度均小于1/4TEa(CLIA’88),且其总精密度小于1/3TEa(CLIA’88)。40例无脂浊血清样本中前白蛋白分别以低速离心、高速离心后经免疫透射比浊法检测后比较,差异无统计学意义(P>0.05)。脂浊对经高速离心处理血标本前白蛋白检测准确性无干扰;对于低速离心处理的血标本,当脂浊指数<43 mg/dL时,不干扰前白蛋白检查效果,当脂浊指数≥43 mg/dL时,会干扰前白蛋白检查效果;高速离心处理的血标本前白蛋白检测结果均高于低速离心标本检测结果(P<0.05)。结论 脂浊对免疫透射比浊法检测前白蛋白水平的准确性存在一定干扰,但轻度脂浊干扰效果并不显著,当脂浊指数≥43 mg/dL时,对前白蛋白干扰效果明显。Objective To investigate the interference effect of lipid turbidity on the detection of prealbumin by Immunotransparency turbidimetry.Methods 40 non-lipid turbidity serum samples from patients with cholesterosis cutis were selected from February 2021 to January 2024 in Shanghai Fengxian District Dermatology Prevention and Control Institute,an additional 40 samples of turbid serum from patients with xanthelasma were selected.Immunotransmission turbidimetry was used to measure prealbumin levels in 80 serum samples,and then the method repeatability of 40 serum samples without lipid turbidity was analyzed,and the lipid turbidity interference effect of 40 lipid turbidity serum samples was analyzed.Results The intra-batch precision of prealbumin was less than 1/4TEa(CLIA’88),and the total precision was less than 1/3TEa(CLIA’88).There was no significant difference in prealbumin in 40 fat-free turbid serum samples after low-speed centrifugation and high-speed centrifugation followed by immunotransmission nephelometry,respectively(P>0.05).Lipid turbidity did not interfere with the accuracy of prealbumin detection in blood samples treated by high-speed centrifugation.For blood samples treated by low-speed centrifugation,when the lipid turbidity index was<43 mg/dL,it did not interfere with the effect of prealbumin examination,and when the lipid turbidity index was≥43 mg/dL,it interfered with the effect of prealbumin examination.The detection result of prealbumin in blood samples treated by high-speed centrifugation was higher than that of low-speed centrifugation samples(P<0.05).Conclusion Lipid turbidity interferes with the accuracy of albumin level before immunotransmission turbidimetry,but the interference effect of mild lipid turbidity is not significant.When the lipid turbidity index is≥43 mg/dL,the interference effect on prealbumin is obvious.

关 键 词：免疫透射比浊法 脂浊 前白蛋白 干扰 

分 类 号：R446.6[医药卫生—诊断学]