高效液相色谱法测定6%苄嘧磺隆·丙草胺·噁草酮颗粒剂中3种有效成分含量  

Determination of 3 activecomponents in 6%Benzyl Propionate Oxadiazon Granules by high performance liquid chromatography

作　　者：张平 杨明君 朱嵘 赵瑜 徐勇存 金鑫雷 ZHANG Ping;YANG Ming-Jun;ZHU Rong;ZHAO Yu;XU Yong-Cun;JIN Xin-Lei(Testing center of Zhejiang Tianfeng bioscience Co.,Ltd.,Jinhua 321000,China)

机构地区：[1]浙江天丰生物科学有限公司检测中心,金华321000

出　　处：《实验室检测》2024年第12期14-17,共4页Laboratory Testing

摘　　要：目的 为了做好企业生产产品的质量把控,提高检测效率,特建立苄嘧磺隆·丙草胺·噁草酮颗粒剂的高效液相色谱方法。方法 采用高效液相色谱法,使用色谱柱ZORBAX Eclipse Plus C_(18)(4.6 mm×50 mm,1.8μm,600 Bar),以乙腈、异丙醇和0.05%磷酸水溶液为流动相,在245 nm波长下对苄嘧磺隆·丙草胺·噁草酮颗粒剂进行液相色谱分析。结果 经检测苄嘧磺隆、丙草胺和噁草酮在此分析方法下不仅具有较高的色谱峰纯度,同时也具有良好的线性关系。该产品中苄嘧磺隆峰纯度992,回收率98.82%;丙草胺峰纯度993.3,回收率99.88%;草酮峰纯度999.3,回收率99.22%。结论 该色谱方法稳定,适合该产品的质量控制分析。Objective In order to ensure the quality control of enterprise production products and improve detection efficiency,a high-performance liquid chromatography method for Benzyl Propionate Oxadiazon Granules is established.Methods The HPLC method was used to separate and determine the contents of Benzyl Propionate Oxadiazon Granules which contained the active ingredients mentioned above.In the method,the column ZORBAX Eclipse Plus C_(18)(4.6 mm×50 mm,1.8μm,600 Bar)was used and the mixture of acetonitrile,isopropanol and 0.05%phosphoric acid solution was used as the mobile phase,and 245 nm was the selected wavelength in the determination.Results It has been found that bensulfuron methyl,pretilachlor,and oxadiazon not only have high chromatographic peak purity under this analytical method,but also have a good linear relationship.The peak purity of bensulfuron methyl in this product is 992,with a recovery rate of 98.82%.The peak purity of pretilachlor is 993.3,with a recovery rate of 99.88%.The peak purity of oxadiazon is 999.3,and the recovery rate is 99.22%.Conclusion This chromatographic method is stable and suitable for quality control analysis of this product.

关 键 词：苄嘧磺隆 丙草胺 噁草酮 高效液相色谱法 定性定量检测 

分 类 号：O657.72[理学—分析化学] TQ457[理学—化学]