高效液相色谱–串联质谱法同时测定心通口服液中9种关键成分的含量  

High-performance liquid chromatography-tandem mass spectrometry for simultaneous determination of nine components in Xintong Oral Liquid

作　　者：耿征 胡丹东 GENG Zheng;HU Dan-Dong(Market Supervision Inspection and Testing Monitoring Center of Yanqing District,Beijing,Beijing 102100,China)

机构地区：[1]北京市延庆区市场监管检验检测监控中心,北京102100

出　　处：《实验室检测》2024年第12期6-9,共4页Laboratory Testing

基　　金：北京市延庆区金桥工程种子资金项目:基于UHPLC-MS法快速检测调节三高类保健食品中非法添加药物方法的建立(NO.24005)

摘　　要：目的 探讨高效液相色谱–串联质谱法同时测定心通口服液中9个成分的含量。方法 采用Phenomenex Luna-C18色谱柱(250 mm×4.6 mm,5μm),乙腈–甲醇–0.02 mol/L乙酸铵为流动相,梯度洗脱,体积流量0.9 mL/min。质谱条件:ESI源,MRM模式,定量离子对峰面积进行定量。结果 阿魏酸、二苯乙烯苷、葛根素等9种成分的线性范围为15.22~15220 ng/mL至25.36~25360 ng/mL。加样回收率分别为98.15%、101.84%,RSD为0.67%~1.64%。9批样品中,9种指标成分质量浓度范围为0.180~4.699 mg/mL。结论 本研究创新运用高效液相色谱–串联质谱法技术,高效精准地测定心通口服液九大核心成分,简化了流程,加速了质量控制,助力标准化生产。Objective To explore the simultaneous determination of the contents of nine components in Xintong Oral Liquid using high-performance liquid chromatography-tandem mass spectrometry.Methods A Phenomenex Luna-C18 column(250 mm×4.6 mm,5μm)was used with acetonitrile-methanol-0.02mol/L ammonium acetate as the mobile phase for gradient elution at a flow rate of 0.9 mL/min.Mass spectrometry conditions:ESI source,MRM mode,quantification was performed using the peak area of the quantitative ion pairs.Results The linear ranges of the nine components,including ferulic acid,diphenylethylene glycoside,puerarin,etc.,were from 15.22 to 15220 ng/mL to 25.36 to 25360 ng/mL.The recovery rates were from 98.15%to 101.84%,with RSDs ranging from 0.67%to 1.64%.In nine batches of samples,the mass concentration ranges of the nine index components were from 0.180 to 4.699 mg/mL.Conclusion This study innovatively applies the HPLC-MS/MS technique to efficiently and accurately determine the nine core components in Xintong Oral Liquid,simplifying the process,accelerating quality control,and aiding in standardized production.

关 键 词：高效液相色谱–串联质谱法 心通口服液 成分含量 

分 类 号：O657.63[理学—分析化学] TQ460.72[理学—化学]