Biogeographic shifts in the microbial co-occurrence network features of three domains across complex environmental gradients in subtropical coastal waters  

作　　者：Dandi Hou Huizhen Yan Huaying Lin Huajun Zhang Demin Zhang Kai Wang 

机构地区：[1]Key Laboratory of Aquaculture Biotechnology of the Ministry of Education,School of Marine Sciences,Ningbo University,Ningbo,China [2]Collaborative Innovation Center for Zhejiang Marine High-Efciency and Healthy Aquaculture,Ningbo,China

出　　处：《Ecological Processes》2024年第4期184-199,共16页生态过程(英文)

基　　金：supported by the National Natural Science Foundation of China(41977192);Zhejiang Provincial Natural Science Foundation of China(LY21D060004);Natural Science Foundation of Ningbo(2021J060 and 2019A610449);Fundamental Research Funds for the Provincial Universities of Zhejiang(SJLY2022001);K.C.Wong Magna Fund in Ningbo University

摘　　要：Background Bacteria,Archaea,and Microeukaryotes comprise taxonomic domains that interact in mediating biogeochemical cycles in coastal waters.Many studies have revealed contrasting biogeographic patterns of community structure and assembly mechanisms in microbial communities from diferent domains in coastal ecosystems;however,knowledge of specifc biogeographic patterns on microbial co-occurrence relationships across complex coastal environmental gradients remains limited.Using a dense sampling scheme at the regional scale,SSU rRNA gene amplicon sequencing,and network analysis,we investigated intra-and inter-domain co-occurrence relationships and network topology-based biogeographic patterns from three microbial domains in coastal waters that show environmental gradients across the inshore-nearshore-ofshore continuum in the East China Sea.Results Overall,we found the highest complexity and connectivity in the bacterial network,the highest modularity in the archaeal network,and the lowest complexity,connectivity,and modularity in the microeukaryotic network.Although microbial co-occurrence networks from the three domains showed distinct topological features,they exhibited a consistent biogeographic pattern across the inshore-nearshore-ofshore continuum.Specifcally,the nearshore zones with intermediate levels of terrestrial impacts refected by multiple environmental factors(including water temperature,salinity,pH,dissolved oxygen,and nutrient-related parameters)had a higher intensity of microbial co-occurrence for all three domains.In contrast,the intensity of microbial co-occurrence was weaker in both the inshore and the ofshore zones at the two ends of the environmental gradients.Archaea occupied a central position in the microbial inter-domain co-occurrence network.In particular,members of the Thaumarchaeota Marine Group I(MGI,now placed within the Family Nitrosopumilaceae of the Phylum Thermoproteota)appeared to be the hubs in the biogeographic shift between inter-domain network modules across environmental grad

关 键 词：CO-OCCURRENCE BIOGEOGRAPHY Bacteria ARCHAEA Microeukaryote Environmental gradient 

分 类 号：Q938.8[生物学—微生物学]