Mn_(3)O_(4)纳米颗粒的类漆酶活性及其对吖啶橙的降解性能研究  

Laccase-like activity of Mn_(3)O_(4) nanoparticles and its application in degrading acridine orange

作　　者：韩倩 葛志强[1] HAN Qian;GE Zhiqiang(School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China)

机构地区：[1]天津大学化工学院,天津300072

出　　处：《化学工业与工程》2025年第1期59-68,共10页Chemical Industry and Engineering

摘　　要：为了构建具有漆酶活性的无机纳米酶以克服天然漆酶的提取纯化过程复杂、稳定性较差和成本较高的缺点,采用化学沉淀法制备了Mn_(3)O_(4)纳米颗粒,以2,4-二氯苯酚为底物对其类漆酶活性进行了评估,并探究了该纳米颗粒对水中吖啶橙的降解能力。结果显示Mn_(3)O_(4)纳米颗粒具有类似漆酶的氧化还原活性,酶动力学实验结果显示在相同的质量浓度下,Mn_(3)O_(4)纳米酶与天然漆酶相比具有更高的催化效率;此外,Mn_(3)O_(4)纳米酶具有更宽的温度耐受范围(25~75℃),室温储存稳定性显著高于天然漆酶。Mn_(3)O_(4)纳米颗粒在氧化还原介质HBT存在下可以快速高效去除水溶液中的有色染料吖啶橙,5 min内的去除效率可达70%。研究结果表明,Mn_(3)O_(4)作为漆酶类纳米酶,催化活性较高,储存稳定,制备过程简单,成本低廉,在染料废水处理领域具有良好的应用前景。Natural laccase suffers from several inherent drawbacks,such as complex purification process,poor stability and high cost,significantly constraining their practical applications.The construction of inorganic nanozymes with laccase activity is a promising strategy to overcome these limitations.In this study,Mn_(3)O_(4) nanoparticles were prepared using a chemical precipitation method.The laccase-like activity was evaluated using 2,4-dichlorophenol as the substrate.Additionally,the removal performance of the nanoparticles towards AO in aqueous solution was investigated.This study shows that Mn_(3)O_(4) nanoparticles have redox activity similar to laccase,and enzyme kinetics experiments demonstrate that Mn_(3)O_(4) nanozyme has higher catalytic efficiency than natural laccase at the same concentration.Furthermore,Mn_(3)O_(4) nanozyme have a wider temperature(25—75℃)and pH(6—7)tolerance range,and the storage stability at room temperature is significantly higher than that of natural laccase.In the presence of the redox mediator HBT,Mn_(3)O_(4) nanoparticles can swiftly and effectively remove AO from aqueous solution,with a removal efficiency of 70%in just 5 min.Therefore,Mn_(3)O_(4),as a laccases-like nanozyme,has excellent developing potential in treatment of dye wastewater due to high catalytic activity,superior storage stability,simplicity of preparation and low cost.

关 键 词：Mn_(3)O_(4)纳米颗粒 漆酶 纳米酶 吖啶橙 降解 

分 类 号：X703[环境科学与工程—环境工程] TQ426[化学工程] TB383.1[一般工业技术—材料科学与工程]