两种免疫学方法检测HBV感染血清标志物与慢性乙型肝炎HBV-DNA定量相关性研究  

Correlation between two immunological methods for detecting serum markers of HBV infection and quantification of HBV-DNA in chronic hepatitis B

作　　者：焦何青 杨艳萍 李莎[1] 蔡鹏虎 李翔[1] JIAO He-Qing;YANG Yan-Ping;LI Sha;CAI Peng-Hu;LI Xiang(Hongxing Hospital of the 13th Division of Xinjiang Production and Construction Corps,Hami 839000,China)

机构地区：[1]新疆生产建设兵团第十三师红星医院,哈密839000

出　　处：《实验室检测》2024年第10期122-124,共3页Laboratory Testing

基　　金：2024年新疆生产建设兵团十三师新星市科学技术局规划课题“两种免疫学方法检测HBV感染血清标志物与慢性乙型肝炎HBV-DNA定量相关性研究”(课题批准号:2024E9)的研究成果

摘　　要：目的研究化学发光免疫分析(CLIA)与酶联免疫吸附试验(ELISA)在HBV感染患者血清标志物检测中的准确性,并分析血清标志物与乙型肝炎病毒脱氧核糖核酸(HBV-DNA)的定量相关性。方法将本院2023年1月—2024年1月期间收治的86例乙型肝炎患者作为研究对象,采用两种免疫学检测方法检测患者的HBV血清标志物,分析两种检测方法的准确性,并采用实时荧光聚合酶链式反应(qRT-PCR)检测进行HBV-DNA载量定量分析。采用McNemar’s检验对血清标志物与HBV-DNA的定量相关性进行分析。结果两种检测方式在HBsAg、HBsAb和HBeAg阳性率检测中的一致性为中等,在HBeAb和HBcAb阳性率检测中的一致性为较强;以ELISA作为金标准,CLIA检测的敏感度为97.30%,特异度为75.00%,以CLIA作为金标准,ELISA检测的敏感度为96.00%,特异度为81.82%;6种血清模式中,乙肝大三阳患者的HBV-DNA阳性率为95.65%,HBV-DNA定量平均值1.12×10^(8) copies/mL,显著高于其他5种模式;小三阳的HBV-DNA阳性检出率也相对较高(65.63%)。结论两种检测方式在HBV感染血清标志物检测中具有较强的一致性,但是CLIA检测的敏感度高于ELISA检测。不同血清模式患者HBV-DNA的阳性率和HBV-DNA定量结果都存在较大差异性。Objective To investigate the accuracy of chemiluminescence immunoassay(CLIA)and enzyme-linked immunosorbent assay(ELISA)in the detection of serum markers in patients with HBV infection,and to analyze the quantitative correlation between serum markers and hepatitis B virus deoxyribonucleic acid(HBV-DNA).Methods A total of 86 patients with hepatitis B admitted to our hospital from January 2023 to January 2024 were selected as subjects.Two immunological detection methods were used to detect HBV serum markers,and the accuracy of the two detection methods was analyzed.Real-time fluorescent polymerase chain reaction(qRT-PCR)was used for quantitative analysis of HBV-DNA load.McNemar's test was used to analyze the quantitative correlation between serum markers and HBV-DNA.Results The positive rates of HBsAg,HBsAb and HBeAg were moderate,but the positive rates of HBeAb and HBcAb were strong.With ELISA as the gold standard,the sensitivity and specificity of CLIA assay were 97.30%and 75.00%,while with CLIA as the gold standard,the sensitivity and specificity of ELISA assay were 96.00%and 81.82%.Among the 6 serum models,the positive rate of HBV-DNA was 95.65%,and the mean quantity of HBV-DNA was 1.12×10^(8) copies/mL,which was significantly higher than that of the other 5 models.The positive rate of HBV-DNA in Xiaosanyang was also relatively high(65.63%).Conclusion The two methods have strong consistency in detecting serum markers of HBV infection,but the sensitivity of CLIA is higher than that of ELISA.The positive rate of HBV-DNA and the quantitative results of HBV-DNA in patients with different serum patterns were significantly different.

关 键 词：慢性乙型肝炎 化学发光免疫分析 酶联免疫吸附试验 检测 乙型肝炎病毒脱氧核糖核酸 相关性 

分 类 号：R512.62[医药卫生—内科学] R446.6[医药卫生—临床医学]