细菌性腹泻患儿粪便标本病原微生物检测及耐药情况分析  

Detection of pathogenic microorganisms and analysis of drug resistance in children with bacterial diarrhea

作　　者：张佳乐 ZHANG Jia-Le(Shanghai Chongming District Center for Disease Control and Prevention,Shanghai 202150,China)

机构地区：[1]上海市崇明区疾病预防控制中心,上海202150

出　　处：《实验室检测》2024年第10期110-112,共3页Laboratory Testing

摘　　要：目的探究发生细菌性腹泻患儿配合采集粪便标本后的病原微生物检测结果与药敏结果。方法2020年4月—2023年6月,我院有170例细菌性腹泻患儿参与研究,家属对研究知情,辅助患儿完成粪便标本采集,标本全部送入检验科实施病原学培养,统计病原菌分布情况,并对病原菌耐药性展开分析。结果170例患儿的粪便样本分离出122株病原菌,革兰阴性菌检出率76.23%,以沙门菌(27.87%)、致泻性大肠埃希菌(21.31%)、志贺菌(21.31%)为主;革兰阳性菌检出率23.77%,以金黄色葡萄球菌(22.95%)为主;沙门菌的四环素(41.18%)、环丙沙星(38.24%)、氨苄西林(35.29%)耐药率最大,致泻性大肠埃希菌的环丙沙星(46.15%)、四环素(38.46%)耐药率最大,志贺菌的环丙沙星(53.85%)、四环素(46.15%)耐药率最大,金黄色葡萄球菌的克林霉素(53.57%)、青霉素(53.57%)耐药率最大。结论沙门菌、致泻性大肠埃希菌、志贺菌与金黄色葡萄球菌是诱发儿童细菌性腹泻的主要原因,明确耐药情况,选择敏感药物治疗,有利于患儿早期恢复健康。Objective To explore the pathogenic microbial detection results and drug susceptibility results of children with bacterial diarrhea after the collection of stool samples.Methods From April 2020 to June 2023,170 children with bacterial diarrhea participated in the study.The family members were informed about the study,and assisted the children to complete the collection of stool specimens.All the specimens were sent to the laboratory for pathogen culture,counted the distribution of pathogens,and analyzed the drug resistance of pathogens.Results 170 stool samples isolated 122 pathogenic strains,The detection rate of gram-negative bacteria was 76.23%,mainly Salmonella(27.87%),diarrheagenic Escherichia coli(21.31%),Shigella(21.31%);The detection rate of gram-positive bacteria was 23.77%,mainly Staphylococcus aureus(22.95%);Most resistance to tetracycline(41.18%),ciprofloxacin(38.24%),ampicillin(35.29%),The greatest resistance to ciprofloxacin(46.15%),tetracycline(38.46%),of ciprofloxacin(53.85%),tetracycline(46.15%),Staphylococcus aureus had to clindamycin(53.57%)and penicillin(53.57%).Conclusion Salmonella,Escherichia coli,Shigella and Staphylococcus aureus are the main causes of bacterial diarrhea in children.The drug resistance and the choice of sensitive drugs are conducive to the early recovery of children.

关 键 词：细菌性腹泻 沙门菌 致泻性大肠埃希菌 志贺菌 金黄色葡萄球菌 病原微生物检测 

分 类 号：R446.5[医药卫生—诊断学] R725.7[医药卫生—临床医学]