胃肠安通过RUFY3抑制胃癌侵袭转移的机制研究  

Inhibitory Effect of Weichang'an(胃肠安)on Invasion and Metastasis of Gastric Cancer by RUFY3

作　　者：李佳 姬高 张光涛 刘怀民 LI Jia;JI Gao;ZHANG Guangtao;LIU Huaimin(Affiliated Cancer Hospital of Zhengzhou University and Henan Cancer Hospital,Zhengzhou 450003,Henan,China;Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200023,China)

机构地区：[1]郑州大学附属肿瘤医院,河南省肿瘤医院,河南郑州450003 [2]上海中医药大学附属龙华医院,上海200023

出　　处：《辽宁中医杂志》2024年第5期148-151,222,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金联合项目(U2004105);河南省自然科学基金面上项目(202300410450);河南省重点研发与推广专项(212102310342);河南省医学科技攻关省部共建项目(SBGJ202103032);河南省中医药科学研究专项课题(2022ZY1224)

摘　　要：目的 研究健脾复方胃肠安抑制胃癌细胞侵袭迁移的机制。方法 CCK8检测胃肠安对胃癌MGC803细胞增殖的影响。采用细胞侵袭迁移实验检测胃肠安和转化生长因子β1(TGF-β1)干预后MGC803细胞迁移和侵袭能力的变化。Western blot检测胃肠安和TGF-β1干预后各组细胞EMT相关蛋白表达情况。荧光双染法检测RUFY3和F-actin表达情况。结果 胃肠安抑制MGC803细胞的增殖呈时间浓度依赖性,48 h和72 h的半数抑制浓度(IC50值)分别为856、781 mg·L^(-1)。TGF-β1可以诱导胃癌MGC803细胞EMT的发生。胃肠安能够逆转TGF-β1对MGC803细胞运动的促进作用。胃肠安抑制了EMT模型中侵袭迁移和MMP家族相关蛋白的表达,并且胃肠安抑制MGC803细胞侵袭力与降低RUFY3的表达相关。结论 胃肠安能够通过RUFY3抑制胃癌MGC803细胞侵袭迁移。Objective To study the mechanism of spleen-strengthening traditional Chinese medicine(TCM)prescriptions Weichang'an(胃肠安)inhibiting the invasion and migration of gastric cancer cells.Methods CCK8 was used to detect the effect of Weichang'an on the proliferation of gastric cancer MGC803 cells.Cell invasion and migration experiment was used to detect the changes of migration and invasion ability of MGC803 cells after the intervention of Weichang'an and transforming growth factor-β1(TGF-β1).The expression of EMT-related proteins in each group was detected through Western blot.Phalloidin staining was used to detect the expressions of RUN and FYVE domain protein 3(RUFY3)and F-actin.Results The proliferation of MGC803 cells was time-dependent with the IC50 of 856 and 781 mg·L^(-1) at 48 h and 72 h,respectively.TGF-a1 can induce EMT in gastric cancer MGC803 cells.Weichang'an reversed the promoting effect of TGF-a1 on the motility of MGC803 cells.The invasion and migration of MGC803 cells and the expression of MMP family related proteins were inhibited in EMT model,and the inhibition of invasion of MGC803 cells was correlated with the decrease of RUFY3 expression.Conclusion The invasion and migration of MGC803 cells in gastric cancer was inhibited by RUFY3.

关 键 词：胃癌 胃肠安 RUFY3 侵袭迁移 

分 类 号：R256.3[医药卫生—中医内科学]