双层CRI污水厂沿程菌群演变规律  

Bacterial Community Succession Pattern in Wastewater Plant Containing Two-layer CRI

作　　者：孟旭 邢涛 佟洪金 张韵洁 雍毅 侯江 林康 林蕊 吴迪 MENG Xu;XING Tao;TONG Hongjin;ZHANG Yunjie;Yong Yi;HOU Jiang;LIN Kang;LIN Rui;WU Di(Sichuan Province Environmental Protection Science Research Institute,Chengdu 610045,China;State Environmental Protection Key Laboratory of Synergetic Control and Joint Remediation for Soil&Water Pollution.College of Ecology and Environment,Chengdu University of Technology,Chengdu 610059,China)

机构地区：[1]四川省生态环境科学研究院,四川成都610045 [2]成都理工大学生态环境学院,国家环境保护水土污染协同控制与联合修复重点实验室,四川成都610059

出　　处：《环境科学与技术》2024年第S2期105-114,共10页Environmental Science & Technology

基　　金：四川省科技计划项目-重点研发项目(2019YFS0060);四川省科技计划项目(2022JDRC0105);四川省生态环境科学研究院财政专项资金项目(ZX-2023060);成都市重点、研发支撑计划(2024-YF05-02242-SN)

摘　　要：人工快速渗滤系统(CRI)广泛用于污水深度处理。CRI与前端单元污水联通,沿程菌群因环境条件的不同而改变,但目前仍缺乏相关研究,无法科学地指导强化菌剂的添加。该文以某污水厂各工艺单元沿程菌群为研究对象,采用高通量测序对菌群演替进行研究。结果表明:高浓度污水和溶氧将降低菌群丰度和多样性,而丰度大于5%的优势菌总和在菌群中比例呈相反趋势。CRI从表层到底层的不同深度菌群,也表现出类似趋势。前端污水约62.2%属细菌可进入CRI-1,CRI-1可分化37.8%的属细菌,CRI-2进一步分化17.4%的属细菌。部分优势菌呈现明显的演替规律:(1)各单元均存在的属细菌为Thauera、norank_f_Anaerolineaceae和unclassified_f_Comamonadaceae,前两者丰度高而后者低;(2)沿程丰度快速下降为Ottowia,慢速下降为norank_f_Synergistaceae;(3)沿程丰度快速增加的为Truepera,而慢速增加的为nonrank_c_Acidobacteria和nonrank_f_Nitrosomonadaceae。该文强调在菌剂接种和挂膜活化时,应根据沿程菌群演变规律针对性添加。The artificial rapid infiltration system(CRI)is widely used for advanced treatment of wastewater in sewage plants.The connectivity between CRI and various front-end treatment units allows for the variation of bacteria along the process,which can be influenced by environmental conditions.However,there is currently a lack of relevant research to scientifically guide the addition of fortified bacteria.agents.Therefore,this study focuses on the microbial community along each unit of a sewage plant and investigates the patterns of microbial community changes using high-throughput sequencing.The results show that high concentrations of wastewater and dissolved oxygen will reduce both the abundance and diversity of microbial communities,but the proportion of dominant bacteria with abundance greater than 5%in the microbial communities shows an opposite trend.Similar trends are also observed in different depths within the CRI system itself.In the front-end processing unit,approximately 62.2%of bacterial species can enter CRI-1,while CRI-1 itself can differentiate into 37.8%of bacterial species,CRI-2 further generates a differentiation of 17.4%of bacterial species.some dominant bacteria exhibit clear patterns.Thauera,norank_f_Anaerolineaceae and unclassified_f_Comamonadaceae are stably present in all units but with higher abundance for Thauera and norank_f_Anaerolineaceae.Ottowia rapidly decreases in abundance as it enters into CRI while norank_f_Synergistaceae decreases slowly.Truepera rapidly increases in abundance as it enters into CRI while nonrank_c_Acidobacteria and nonrank_f_Nitrosomonadaceae increase slowly.The paper emphasizes that targeted addition of bacterial agents should be made based on the evolutionary trends of the microbial community along the way during the inoculation and colonization activation.

关 键 词：高通量测序 人工快渗系统 细菌丰度 多样性指数 强化菌剂 

分 类 号：X172[环境科学与工程—环境科学]