乳胶增强免疫散射比浊法、免疫层析法、免疫比浊法检测超敏C反应蛋白的性能比较  

Comparison of the Performance of Latex Enhanced Immunoscattering Turbidimetry、Immunochromatography And Immunoturbidimetry for the Detection of Hypersensitive C-reactive Protein

作　　者：张璋 黄静仪 Zhang Zhang;Huang Jingyi(Qingcheng District People's Hospital,Qingyuan Guangdong 511500,China)

机构地区：[1]清远市清城区人民医院,广东清远511500

出　　处：《生命科学仪器》2024年第5期18-20,共3页Life Science Instruments

摘　　要：目的 比较乳胶增强免疫散射比浊法、免疫层析法、免疫比浊法检测超敏C反应蛋白(hs-CRP)的性能。方法 对三种检测方式(生化分析仪、干式免疫分析仪、血细胞分析仪)检测hs-CRP的精密度、准确度、线性等进行分析,以DADE系统为参照进行对比。结果 乳胶增强免疫散射比浊法、免疫层析法、免疫比浊法检测hs-CRP的批间精密度为2.80%~14.51%,低于DADE系统的1.40%。Passing-Bablok回归分析显示,免疫比浊法与DADE系统的相关性好,回归方程为:Y=-0.15+1.01x。免疫层析法、免疫散射比浊法与DADE系统的相关性不佳,斜率分别为1.18、0.73。线性回归分析显示,免疫比浊法的R2为0.998,而免疫层析法与免疫散射比浊法的R^(2)<0.99。免疫比浊法与DADE系统的一致性高于免疫层析法及免疫散射比浊法,P<0.05。结论 乳胶增强免疫散射比浊法、免疫层析法、免疫比浊法检测hs-CRP的性能均符合各自厂商的性能要求,可用于临床检测。Objective:To compare the performance of latex enhanced immunoscattering turbidimetry,immunochromatography and immunoturbidimetry for the detection of hypersensitive C-reactive protein(hs-CRP).Methods:The precision,accuracy and linearity of three detection methods(biochemical analyzer、dry immunoassay analyzer and blood cell analyzer)for detecting hs-CRP were analyzed and compared with DADE system.Results:The accuracy of hs-CRP by emulsion enhanced immunoscattering turbidimetry,immunochromatography and immunoturbidimetry was 2.80%~14.51%,which was lower than that by DADE system.Passing-Bablok regression analysis showed that the correlation between immunoturbidimetry and DADE system was good,and the regression equation was Y=-0.15+1.01x.The correlation between immunochromatography and immunoscattering turbidimetry and DADE system was poor,with slopes of 1.18 and 0.73,respectively.Linear regression analysis showed that the R^(2) of immunoturbidimetry was 0.998,while that of immunochromatography and immunoscattering turbidimetry was less than 0.99.The consistency of immunoturbidimetry and DADE system was higher than that of immunochromatography and immunoscattering turbidimetry,P<0.05.Conclusion:The properties of latex enhanced immunoscattering turbidimetry,immunochromatography and immunoturbidimetry for the detection of hs-CRP meet the requirements of their respective manufacturers and can be used for clinical detection.

关 键 词：乳胶增强免疫散射比浊法 免疫层析法 免疫比浊法 超敏C反应蛋白 

分 类 号：R446[医药卫生—诊断学]