全自动碘分析仪测定尿碘的方法学评价  

Methodological evaluation of measuring urinary iodine using a fully automated iodine analyzer

作　　者：谢莉[1] 赵俊君[2] 张玉华[3] Xie Li;Zhao Junjun;Zhang Yuhua(Department of Physical and Chemical Inspection,Puyang Municipal Center for Disease Control and Prevention,Puyang 457001,China;Office of Puyang Municipal Center for Disease Control and Prevention,Puyang 457001,China;Puyang Municipal Center for Disease Control and Prevention,Puyang 457001,China)

机构地区：[1]濮阳市疾病预防控制中心理化检验科,濮阳457001 [2]濮阳市疾病预防控制中心办公室,濮阳457001 [3]濮阳市疾病预防控制中心,濮阳457001

出　　处：《中华地方病学杂志》2024年第12期1010-1013,共4页Chinese Journal of Endemiology

摘　　要：目的评价全自动碘分析仪测定尿碘方法的可行性。方法应用全自动碘分析仪测定尿碘含量,在0~1200μg/L标准曲线范围内(低浓度标准系列为0~300μg/L、高浓度标准系列为300~1200μg/L),进行标准曲线线性关系、检出限、精密度及准确度(0~300μg/L范围内尿碘标准物质GBW109m、GBW110e,300~1200μg/L范围内尿碘标准物质GBW111m、GBW112h)的方法学评价;并与砷铈催化分光光度法手动测定结果进行方法比对实验。结果全自动碘分析仪测定尿碘在0~1200μg/L标准曲线范围内均有良好的线性关系,│r│均>0.9990;最低检出限为3.17μg/L。其中,0~300μg/L范围内低、中、高3种浓度尿碘标准物质的变异系数分别为6.79%、2.53%、1.20%;加标回收率范围为94.8%~101.2%;300~1200μg/L范围内低、高2种浓度尿碘标准物质的变异系数分别为1.21%、1.62%;加标回收率范围为100.6%~105.1%。尿碘标准物质GBW109m、GBW110e、GBW111m、GBW112h测定结果均在给定标准值±不确定度范围内[(69.7±9.0)、(210±15)、(574±17)、(794±28)μg/L]。应用全自动碘分析仪与砷铈催化分光光度法手动测定结果比较,差异均无统计学意义(均P>0.05)。结论全自动碘分析仪检测方法可全程自动化,操作简单省时,系统准确度高、精密度好,适用于大量尿碘样品检测。Objective To evaluate the feasibility of using a fully automated iodine analyzer to measure urinary iodine.Methods A fully automated iodine analyzer was used to determine the urinary iodine content,a methodological evaluation was conducted on the linear relationship,detection limit,precision,and accuracy(urinary iodine standard substances GBW109m,GBW110e within the range of 0-300μg/L,and GBW111m,GBW112h within the range of 300-1200μg/L)of the standard curve within the ranged from 0 to 1200μg/L(low concentration standard series:0-300μg/L,high concentration standard series:300-1200μg/L).A method comparison experiment with the manual measurement results of arsenic cerium catalytic spectrophotometry was conducted.Results The fully automated iodine analyzer had a good linear relationship for the determination of urinary iodine within the standard curve ranges of 0-1200μg/L,with│r│>0.9990;the minimum detection limit was 3.17μg/L.Among them,the coefficients of variation for low,medium,and high concentrations of urinary iodine standard substances in the range of 0-300μg/L were 6.79%,2.53%and 1.20%,respectively;and the recovery rate ranged from 94.8%to 101.2%.The coefficients of variation for low and high concentrations of urinary iodine standard substances within the range of 300-1200μg/L were 1.21%and 1.62%,respectively;and the recovery rate ranged from 100.6%to 105.1%.The determination results of urinary iodine standard substances GBW109m,GBW110e,GBW111m,and GBW112h were all within the given standard value±uncertainty range[(69.7±9.0),(210±15),(574±17),(794±28)μg/L].There was no statistically significant difference between the results obtained by using a fully automated iodine analyzer and the manual measurement results of arsenic cerium catalytic spectrophotometry(P>0.05).Conclusion The fully automated iodine analyzer detection method can be fully automated,with simple and time-saving operation,high system accuracy,and good precision,suitable for large-scale urine iodine sample detection.

关 键 词：碘 尿 方法学评价 

分 类 号：R446.12[医药卫生—诊断学]