柯萨奇病毒A组6型VP1的主要特性及表位预测分析  

Predictive analysis of main characteristics and epitopes of VP1 from Coxsackievirus A6

作　　者：刘益文 张晓雯 高邦婷 项钦戈 张晨阳 左成 杨欢 汪大巍 张高博 LIU Yiwen;ZHANG Xiaowen;GAO Bangting;XIANG Qinge;ZHANG Chenyang;ZUO Cheng;YANG Huan;WANG Dawei;ZHANG Gaobo(Wuhan Huaxia Institute of Technology,Wuhan 430223,China;Hubei Gedian Humanwell Pharmaceutical Excipients Co.,Ltd.,Ezhou 436070,Hubei,China)

机构地区：[1]武汉华夏理工学院,武汉430223 [2]湖北葛店人福药用辅料有限责任公司,湖北鄂州436070

出　　处：《生物信息学》2025年第1期50-60,共11页Chinese Journal of Bioinformatics

基　　金：武汉华夏理工学院校级科研基金项目(No.22011)

摘　　要：运用生物信息学方法预测分析襄阳地区分离的柯萨奇病毒A组6型(Coxsackievirus A6, CV-A6)病毒蛋白(Viral protein, VP)中VP1的主要特性及表位。应用ProtParam, SOPMA,Phyre 2,DNAstar v8.1.3,Mega11,ABCpred, ElliPro, NetMHCpan-4.1,NetMHCIIpan-4.0等软件和在线网站预测分析CV-A6 VP1包括理化性质、结构特征、序列特点,亲缘关系在内的主要特性及B,T细胞抗原表位。结果发现该分离株VP1为碱性、不稳定的亲水性蛋白,其二级结构以无规则卷曲为主,核苷酸(氨基酸)同源性不一,有12个氨基酸突变位点,属于D3a亚型。该蛋白还存在多个潜在及优势B,T细胞抗原表位。CV-A6襄阳分离株与国内广西、广东地区CV-A6共进化共循环,具有一定的免疫潜能,可为分子流行病学监测以及多价疫苗的研制提供科学依据与参考。To predict and analyze the main characteristics and epitopes of VP1 in the viral protein(VP)of Coxsackievirus A6(CV-A6)isolated from Xiangyang area using bioinformatics methods.The CV-A6 VP1’s main characteristics including physicochemical properties,structural features,sequence characteristics,affinity and B/T cell epitopes are predicted and analyzed by software and online sites such as ProtParam,SOPMA,Phyre 2,DNAstar v8.1.3,Mega11,ABCpred,ElliPro,NetMHCpan-4.1,NetMHCIIpan-4.0.The isolate,VP1,is a basic,unstable,hydrophilic protein which has the secondary structure mainly consists of random coil,disparate nucleotide(amino acid)homology,12 amino acid mutation sites,and belongs to the D3a isoform.There are also multiple potential and dominant B/T cell epitopes for VP1.The CV-A6 Xiangyang isolate co-evolved and co-circulated with CV-A6 in Guangxi and Guangdong,China,and has certain immune potential,which can provide scientific basis and reference for molecular epidemiological surveillance and the development of multivalent vaccines.

关 键 词：CV-A6 VP1 生物信息学 主要特性 表位 

分 类 号：R373.23[医药卫生—病原生物学]