鼠疫耶尔森氏菌单核苷酸基因多态性与适应度的关系分析  

Analysis of relationship between single nucleotide gene polymorphism and fitness of Yersinia pestis

作　　者：廖春瑞 LIAO Chun-Rui(Rodent Control Department,Balinzuoqi Disease Prevention and Control Center,Chifeng 025450,China)

机构地区：[1]巴林左旗疾病预防控制中心鼠防科,赤峰025450

出　　处：《实验室检测》2024年第11期123-126,共4页Laboratory Testing

摘　　要：目的探究鼠疫耶尔森氏菌单核苷酸基因多态性与适应度的关系。方法本文通过体外连续传代共孵育竞争、体外静置竞争,揭示GTG在不同菌株之间竞争中的作用机制。结果两种菌株,随传代次数增加,GTG菌株很快就成为绝对优势,表现出了有竞争性的适应性;GTG菌株与TTG菌株在不同逆境(Asp缺失、弱酸和高盐)的共同培养下,GTG菌株具有更显著的适应能力;巨噬细胞的竞争性存活试验中,GTG菌株与TTG菌株的比例并没有明显改变;细胞内竞争性扩增产物实验,GTG菌株∶TTG菌株=10∶1的小鼠,在给药过程中,平均给药剂量超过了理论值,从而使小鼠提前死亡。结论GTG在不同菌株之间存在竞争适应性。巨噬细胞的细胞内竞争和小鼠体内的竞争实验没有发现GTG菌株和TTG菌株的适应性有显著的差别,但是TTG株在大肠杆菌竞争中体现的适应度优势。Objective The relationship between fitness and single nucleotide gene polymorphism of Yersinia pestis was investigated.Methods In this paper,the mechanism of GTG in competition between different strains was revealed through continuous in vitro co-incubation competition and in vitro static competition.Results With the increase of passage times,GTG strain quickly became the absolute dominant strain and showed competitive adaptability.Under different stresses(Asp deficiency,weak acid and high salt),GTG and TTG strains showed more remarkable adaptability.In the competitive survival test of macrophages,the ratio of GTG strains to TTG strains did not change significantly.In the intracellular competitive amplification product experiment,mice with GTG∶TTG=10∶1,the average dose exceeded the theoretical value during the administration process,resulting in early death of the mice.Conclusion GTG has competitive adaptability among different strains.There was no significant difference between the fitness of GTG and TTG strains in the intracellular competition of macrophages and the competition in mice,but TTG strains showed a fitness advantage in E.coli competition.

关 键 词：鼠疫耶尔森氏菌 基因多态性 适应度 

分 类 号：R378.61[医药卫生—病原生物学]