安胃颗粒的薄层鉴别与优化及有效成分含量测定  

Thin layer identification and optimization of Anwei Granules and determination of active components

作　　者：陈舒茵[1] 梁国成[1] 秦辛[1] 莫钧婷 冯文勇 龙珍 CHEN Shu-Yin;LIANG Guo-Cheng;QIN Xin;MO Jun-Ting;FENG Wen-Yong;LONG Zhen(Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine,Nanning 530011,China;Food and Drug Evaluation and Inspection Center of Guangxi Zhuang Autonomous Region,Nanning 530023,China)

机构地区：[1]广西中医药大学附属瑞康医院,南宁530011 [2]广西壮族自治区食品药品审评查验中心,南宁530023

出　　处：《实验室检测》2024年第11期115-118,共4页Laboratory Testing

基　　金：2021年广西教育厅青年教师基础能力提升项目(2021KY0286)

摘　　要：目的建立专属性强、可靠以及准确的质量控制方法以改善和提高安胃颗粒的质量标准。方法运用硅胶薄层层析色谱法,考察不同试剂的层析系统、温度和湿度以优化方中制黄连、丹参、乌药的薄层定性鉴别;运用紫外–分光光度法(UV-Vis)检测制剂中总生物碱的含量。结果确定制黄连的展开系统为正丁醇–冰醋酸–水(7∶2.5∶2),丹参的展开系统为甲苯–三氯甲烷–乙酸乙酯–甲醇–甲酸(2∶3∶4∶0.5∶2),乌药的展开系统为三氯甲烷–乙酸乙酯–甲酸(4∶3∶0.2),三个药的阴性均无干扰。经UV-Vis测定,该制剂总生物碱含量为4.8328 mg/g,RSD%为1.83%。结论本研究建立了一种能有效提高安胃颗粒质量标准的方法,并具有重复性好、专属性强的特点。Objective To establish a specific,reliable and accurate quality control method to improve the quality standard of Anwei Granules.Methods The chromatography system,temperature and humidity of different reagents were investigated by silica gel thin layer chromatography(TLC)to optimize the thin layer identification of Coptidis rhizoma,Radix salviae miltiorrhizae and Linderae Radix.The content of total alkaloids in the preparation was determined by UVVis.Results The development system of Coptidis rhizoma was determined to be n-butanol-acetic acid-water(7∶2.5∶2),the development system of Radix salviae miltiorrhizae was toluene-trichloromethane-ethyl acetate-methanol-formic acid(2∶3∶4∶0.5∶2),and the development system of Linderae Radix was trichloromethane-ethyl acetate-formic acid(4∶3∶0.2).The negative results of the three drugs were not interfered.The total alkaloid content was 4.8328 mg/g and RSD%was 1.83%by UV-Vis assay.Conclusion In this study,an effective method for improving the quality standard of Anwei Granules was established,which has the characteristics of good repeatability and strong specificity.

关 键 词：安胃颗粒 薄层鉴别 紫外分光光度法 总生物碱 

分 类 号：R286.0[医药卫生—中药学]