实时荧光定量PCR技术定量检测乙型肝炎病毒DNA效果评价  

Evaluation of the effect of real-time fluorescence quantitative technique PCR for quantitative detection of hepatitis B virus DNA

作　　者：郭金芳 GUO Jin-Fang(Department of Laboratory Medicine,Second People’s Hospital of Binzhou City,Binzhou 256800,China)

机构地区：[1]滨州市第二人民医院检验科,滨州256800

出　　处：《实验室检测》2024年第11期164-166,共3页Laboratory Testing

摘　　要：目的观察实时荧光定量PCR检测技术在检测乙型肝炎病毒DNA时的检验结果以及实质性影响。方法研究数据选取2023年1月—2023年12月期间的乙肝患者1500例,均进行血清学标志物法和实时荧光定量PCR检测,对照标准为临床诊断结果,对比指标为DNA定量检测结果。结果共确诊的1500例乙型肝炎患者中,实时荧光定量PCR技术检测出的乙型肝炎病毒DNA定量结果显示,有1380例患者(92.00%)的病毒载量能够被明确量化,而相比之下,血清学标志物法仅能明确量化1260例(84.00%)患者的病毒载量。除技术外,可能影响最终检测结果的还包括实验室、样本和核酸提取等多环节的操作、贮存等因素。结论实时荧光定量PCR技术相较于传统的血清血标志物检测法而言,其敏感性和准确度更高,更加适用于现代检验医学科。Objective To observe the test results and substantial impact of real-time fluorescence quantitative PCR detection technology in detecting hepatitis B virus DNA.Methods 1500 patients with hepatitis B from January 2023 to December 2023 were selected for the study data,and all of them were detected by serological markers and real-time fluorescent quantitative PCR.The control standard was the clinical diagnosis results,and the comparison index was the DNA quantitative detection results.Results Among the 1500 confirmed hepatitis B patients,real-time fluorescence quantitative PCR technology detected hepatitis B virus DNA quantification results,which showed that the viral load of 1380 patients(92.00%)could be clearly quantified,while the serological marker method could only clearly quantify the viral load of 1260 patients(84.00%).In addition to technology,factors that may affect the final test results include laboratory operations,sample and nucleic acid extraction,and storage at multiple stages.Conclusion Real time fluorescence quantitative PCR technology has higher sensitivity and accuracy compared to traditional serum blood marker detection methods,and is more suitable for modern laboratory medicine.

关 键 词：实时荧光定量PCR技术 乙型肝炎病毒 DNA定量检测 影响因素 

分 类 号：R512.62[医药卫生—内科学] R440[医药卫生—临床医学]