宁心缓痛汤对子宫内膜异位症痛经疼痛、细胞焦亡及NLRP3炎性小体的影响  

Effect of Ningxin Huantong Tang on dysmenorrhea pain,cell pyroptosis,and NLRP3 inflammasome in endometriosis

作　　者：司晴 王桂华 张铁征 郑新平 马艳东 SI Qing;WANG Guihua;ZHANG Tiezheng;ZHENG Xinping;MA Yandong(Department of Gynecology,Hengshui Hospital of Traditional Chinese Medicine,Hengshui,Hebei 053000,China)

机构地区：[1]衡水市中医医院妇科,河北衡水053000

出　　处：《安徽医药》2025年第3期482-487,共6页Anhui Medical and Pharmaceutical Journal

基　　金：衡水市科技计划项目(14027A)。

摘　　要：目的探究宁心缓痛汤调控核苷酸结合结构域富含亮氨酸重复序列和含热蛋白结构域受体3(NLRP3)炎性小体介导的细胞焦亡对子宫内膜异位症痛经大鼠疼痛反应的影响。方法2022年1月至2023年5月,采用随机数字表法将50只大鼠分为假手术组10只和造模组40只。造模组在造模过程中有3只大鼠造模失败、1只大鼠死亡,造模成功后的36只大鼠采用随机数字表法分成四组:子宫内膜异位症疼痛大鼠模型组(EMT组)、低剂量宁心缓痛汤干预组(宁心缓痛汤-L组)、高剂量宁心缓痛汤干预组(宁心缓痛汤-H组)和孕三烯酮组,每组各9只。检测各组大鼠痛经状况和血清中前列腺素F2α(PGF2α)和前列腺素E2(PGE2)含量;苏木精-伊红(HE)染色检测异位子宫内膜组织病理学变化;末端脱氧核苷酸转移酶(TUNEL)检测异位子宫内膜组织细胞凋亡;蛋白质印迹法检测异位子宫内膜组织中胱天蛋白酶-1(caspase-1)、NLRP3、Gasdermin D(GSDMD)、白细胞介素(IL)-1β、IL-18蛋白表达。结果和假手术组相比,EMT组大鼠扭体次数、血清中PGF2α含量[(208.91±17.06)ng/L比(115.36±10.27)ng/L]和PGF2α/PGE2(0.97±0.10比0.27±0.03)、异位子宫内膜组织中细胞凋亡率和NLRP3(0.81±0.09比0.15±0.02)、caspase-1(0.73±0.08比0.12±0.02)、GSDMD(0.91±0.11比0.30±0.03)、IL(白细胞介素)-1β(1.21±0.12比0.24±0.03)、IL-18蛋白(0.92±0.10比0.26±0.03)表达明显增加,疼痛潜伏期、血清中PGE2含量[(214.31±19.52)ng/L比(423.78±35.61)ng/L]明显减少(P<0.05);和EMT组相比,宁心缓痛汤-L组、宁心缓痛汤-H组和孕三烯酮组大鼠扭体次数、血清中PGF2α含量和PGF2α/PGE2、异位子宫内膜组织中细胞凋亡率和NLRP3、caspase-1、GSDMD、IL-1β、IL-18蛋白表达明显减少,疼痛潜伏期、血清中PGE2含量明增加(P<0.05)。结论宁心缓痛汤可能通过抑制NLRP3介导的细胞焦亡,减轻炎症反应,调控血清中的PGE2、PGF2α的合成与释放,从而对子宫内�Objective To investigate the effect of Ningxin Huantong Tang on the pain response of endometriosis dysmenorrhea rats by regulating the inflammatory cell death mediated by nucleotide binding oligomerization domain receptor 3(NLRP3)inflammasome.Methods From January 2022 to May 2023,Using a random number table method,50 rats were divided into a sham surgery group of 10 rats and a modeling group of 40 rats.During the modeling process,3 rats failed and 1 rat died.After successful modeling,the 36 rats were randomly divided into four groups using a random number table:endometriosis pain rat model group(EMT group),low-dose Ningxin Huantong Tang intervention group(Ningxin Huantong Tang-L group),high-dose Ningxin Huantong Tang intervention group(Ningxin Huantong Tang-H group),and gestrinone group,with 9 rats in each group.The dysmenorrhea status and serum levels of prostaglandin F2 alpha(PGF2 alpha)and prostaglandin E2(PGE2)in each group of rats were detected;Hematoxylin eosin(HE)staining was used to detect pathological changes in ectopic endometrial tissue;Terminal deoxynucleotide transferase(TUNEL)was used to detect apoptosis in ectopic endometrial tissue cells;Western blotting was used to detect the protein expression of cysteine containing aspartic acid protease(caspase-1),NLRP3,Gasdermin D(GSDMD),interleukin-1β(IL-1β),and interleukin-18(IL-18)in ectopic endometrial tissue.Results Compared with the sham surgery group,the number of body twists,serum PGF2αcontent[(208.91±17.06)ng/L vs.(115.36±10.27)ng/L],PGF2α/PGE2 ratio(0.97±0.10 vs.0.27±0.03),apoptosis rate and NLRP3(0.81±0.09 vs.0.15±0.02),caspase-1(0.73±0.08 vs.0.12±0.02),GSDMD(0.91±0.11 vs.0.30±0.03),IL-1β(1.21±0.12 vs.0.24±0.03),IL-18 protein(0.92±0.10 vs.0.26±0.03)in ec topic endometrial tissue of EMT group were significantly increased.The pain latency and serum PGE2 content[(214.31±19.52)ng/L vs.(423.78±35.61)ng/L]were significantly reduced(P<0.05).Compared with the EMT group,the number of body twists,serum PGF2αcontent and PGF2α/PGE2 ratio

关 键 词：子宫内膜异位症 宁心缓痛汤 核苷酸结合结构域富含亮氨酸重复序列和含热蛋白结构域受体3 细胞焦亡 痛经 疼痛反应 前列腺素 

分 类 号：R285.5[医药卫生—中药学]