鄂西北野生仙鹤草基源鉴定及质量分析研究  

Provenance and quality of the wild agrimony in northwest Hubei

作　　者：张华妮 朱少铭 吴云毅 程刚 赵亮 殷莉 曹贤达 黄美松 杨艳芳 ZHANG Huani;ZHU Shaoming;WU Yunyi;CHENG Gang;ZHAO Liang;YIN Li;CAO Xianda;HUANG Meisong;YANG Yanfang(Department of Pharmacy,Shiyan Hospital of Integrated Traditional Chinese and Western Medicine,Shiyan,Hubei 442000,China;Department of Endocrinology,Shiyan Hospital of Integrated Traditional Chinese and Western Medicine,Shiyan,Hubei 442000,China;College of Pharmacy,Hubei University of Chinese Medicine,Wuhan,Hubei 430065,China)

机构地区：[1]十堰市中西医结合医院药学部,湖北十堰442000 [2]十堰市中西医结合医院内分泌科,湖北十堰442000 [3]湖北中医药大学药学院,湖北武汉430065

出　　处：《安徽医药》2025年第3期507-512,I0002,共7页Anhui Medical and Pharmaceutical Journal

基　　金：湖北省中医药管理局2023~2024年度中医药科研项目(ZY2023F071);全国中药特色技术传承人才培训项目(国中医药人教函〔2023〕96号)。

摘　　要：目的确定鄂西北野生仙鹤草基源及其品质。方法2022年12月至2023年6月观察鄂西北9个产地野生仙鹤草的植物形态,并与中国植物志比对确定其基源;薄层色谱鉴别方法对饮片粉末进行定性鉴别;建立高效液相色谱法(HPLC)测定仙鹤草槲皮素的含量。色谱柱为Agilent 5 HC-C_(18)(250 mm×4.6 mm,5μm),以乙腈-0.2%磷酸水溶液(28∶72,V/V)为流动相;流速1 mL/min;检测波长368 nm;柱温30℃。结果鄂西北9个产地野生仙鹤草指标性成分槲皮素的平均含量为0.75 mg/g,对照药材含量为0.75 mg/g,含量的平均值与对照药材的相当。结论所建立的槲皮素含量测定分析方法准确、可靠,可为后续质量标准的建立提供参考;在9个产地样品中,以茅箭、郧西县和张湾西沟3个产地的槲皮素含量相对较高,跟市场流通饮片槲皮素含量差异较大,可能跟采收时间、产地加工等因素有关。Objective To determine the source and quality of the wild Agrimony(Agrimoniae Herba)in Northwest Hubei Province.Methods Observation of the plant morphology of wild crane grass from nine production areas in northwest Hubei province from December 2022 to June 2023,and compared with the records of Flora of China to determine its source.High-performance liquid chromatography(HPLC)was performed to determine the content of quercetin on Agilent 5 HC-C_(18) column(250 mm×4.6 mm,5μm).Acetonitrile-0.2% phosphoric acid aqueous solution(28∶72,V/V)was used as the mobile phase,with flow rate at 1 mL/min,detection wavelength 368 nm,and column temperature 30℃.Results The average contents of quercetin was 0.75 mg/g in the wild Agrimony and 0.75 mg/g in the control in 9 regions of northwest Hubei,which were similar to each other.Conclusions The content determination method of quercetin is accurate and reliable,which can provide reference for future development of quality standards.Among the samples from the nine producing areas,the contents of quercetin from Maojian,Yunxi County and Xigou of Zhangwan are relatively high,which is quite different from that sold in the market.It may be affected by the factors such as harvest time and processing technique in the producing areas.

关 键 词：仙鹤草 基源鉴定 仙鹤草酚B 槲皮素 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]