慢性阻塞性肺疾病急性加重病人血清组蛋白H3与外周血NLRP3炎症小体表达的相关性  

Correlation between serum histone H3 and peripheral blood NLRP3 inflammasome expression in patients with AECOPD and its clinical significance

作　　者：邵宪萍 金小乐 张丹 乔缘 任慧敏[1] 高飞 SHAO Xianping;JIN Xiaole;ZHANG Dan;QIAO Yuan;REN Huimin;GAO Fei(Department of Emergency,The First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000,China)

机构地区：[1]河北北方学院附属第一医院急诊科,河北张家口075000

出　　处：《安徽医药》2025年第3期570-574,共5页Anhui Medical and Pharmaceutical Journal

基　　金：河北省医学科学研究基金项目(20231451);河北省2019年度医学科学研究课题计划项目(20190902)。

摘　　要：目的研究慢性阻塞性肺疾病急性加重(AECOPD)病人血清组蛋白H3与外周血Nod样受体蛋白3(NLRP3)炎症小体表达的相关性及其临床意义。方法选择2020年1月至2021年5月河北北方学院附属第一医院收治的AECOPD病人作为AECOPD组(n=105)、稳定期慢性阻塞性肺疾病(COPD)病人作为稳定期COPD组(n=120),另取同期体检的健康志愿者作为健康组(n=100)。检测血清组蛋白H3含量及外周血NLRP3、凋亡相关斑点样蛋白(ASC)、胱天蛋白酶-1(caspase-1)的mRNA相对表达水平;随访AECOPD病人出院后1年的预后情况。结果AECOPD组的血清组蛋白H3含量及外周血NLRP3、ASC、caspase-1表达水平均高于稳定期COPD组及健康组[(8.39±2.26)μg/L比(6.11±1.58)μg/L、(3.42±0.94)μg/L,1.40±0.41比(1.12±0.37、1.00±0.24),1.28±0.24比(1.12±0.32、1.00±0.27),1.21±0.28比(1.11±0.30、1.00±0.22)](P<0.05);AECOPD组中预后不良病人的血清组蛋白H3含量及外周血NLRP3、ASC、caspase-1表达水平均高于预后良好病人[(10.27±1.66)μg/L比(7.41±1.88)μg/L,1.49±0.32比1.38±0.41,1.40±0.35比1.21±0.27,1.33±0.24比1.14±0.28](P<0.05);血清组蛋白H3含量及外周血ASC、caspase-1表达水平对AECOPD具有诊断价值、对AECOPD预后具有评估价值。结论AECOPD发病过程中组蛋白H3释放增加与NLRP3炎症小体激活可能共同参与了AECOPD的发病及预后不良。Objective To study the correlation between serum histone H3 and peripheral blood Nod-like receptor protein 3(NLRP3)inflammasome expression in patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD)and its clinical significance.Methods One hundred and five patients with AECOPD treated in the First Affiliated Hospital of Hebei North University from January 2020 to May 2021 were selected as AECOPD group,120 patients with stable chronic obstructive pulmonary disease(COPD)as stable COPD group,and healthy volunteers who underwent physical examination at the same time as the healthy group.The content of serum histone H3 and the relative mRNA expression levels of NLRP3,apoptosis-associated speck-like protein containing a CARD(ASC)and cysteinyl aspartate specific proteinase-1(caspase-1)in peripheral blood were detected.The prognosis of AECOPD patients one year after discharge was followed up.Results The content of serum histone H3 and the expression levels of NLRP3,ASC and caspase-1 in peripheral blood of AECOPD group were higher than those of stable COPD group and healthy group[(8.39±2.26)μg/L vs.(6.11±1.58)μg/L,(3.42±0.94)μg/L,1.40±0.41 vs.(1.12±0.37,1.00±0.24),1.28±0.24 vs.(1.12±0.32,1.00±0.27),1.21±0.28 vs.(1.11±0.30,1.00±0.22)](P<0.05).In AECOPD group,the content of serum histone H3 and the expression levels of NLRP3,ASC and caspase-1 in peripheral blood of patients with poor prognosis were higher than those of patients with good prognosis[(10.27±1.66)μg/L vs.(7.41±1.88)μg/L,1.49±0.32 vs.1.38±0.41,1.40±0.35 vs.1.21±0.27,1.33±0.24 vs.1.14±0.28].The content of serum histone H3 and the expression levels of ASC and caspase-1 in peripheral blood had diagnostic value and prognostic evaluation value for AECOPD.Conclusion The increased release of histone H3 and the activation of NLRP3 inflammasome in the pathogenesis of AECOPD may be involved in the pathogenesis and poor prognosis of AECOPD.

关 键 词：慢性阻塞性肺疾病急性加重期 组蛋白H3 Nod样受体蛋白3 预后 

分 类 号：R563.9[医药卫生—呼吸系统]