Effects of acupuncture on SATB1/p21 signaling pathway and SASPs in MPTP-induced Parkinson disease model mice  

针刺对MPTP诱导的帕金森病模型小鼠SATB1/p21信号通路和衰老相关分泌表型的影响

作　　者：LI Guona ZHAO Chen WANG Zhaoqin HUA Xuegui WU Luyi ZHANG Xiyin SHEN Lin CHEN Ziyi HU Wenqing CHEN Yiyi CHENG Ling QIAO Yu 李国娜;赵琛;王照钦;华雪桂;吴璐一;张惜音;沈琳;陈子怡;胡文清;陈奕奕;程玲;乔宇(Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;School of Acupuncture-moxibustion and Tuina,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Research Institute of Acupuncture and Meridian,Shanghai University of Traditional Chinese Medicine,Shanghai 200030,China;East Hospital Affiliated to Tongji University,Shanghai 200120,China)

机构地区：[1]Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China [2]School of Acupuncture-moxibustion and Tuina,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China [3]Shanghai Research Institute of Acupuncture and Meridian,Shanghai University of Traditional Chinese Medicine,Shanghai 200030,China [4]East Hospital Affiliated to Tongji University,Shanghai 200120,China

出　　处：《Journal of Acupuncture and Tuina Science》2025年第1期1-11,共11页针灸推拿医学(英文版)

基　　金：国家中医药管理局高水平中医药重点学科建设项目,No.zyyzdxk-2023068;国家自然科学基金项目,No.82205260;上海市浦东新区卫计委名中医培养计划,No.PWRzm2020-12;浦东新区卫计委学科建设中医针灸重点专科,No.PWZzk2022-25.

摘　　要：Objective:To observe the effects of acupuncture on the motor function of Parkinson disease(PD)model mice and to investigate the neuroprotective effects of acupuncture on PD from the perspective of cellular senescence.Methods:C57BL/6J mice were randomly divided into a normal control(NC)group,a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)group,an acupuncture(ACU)group,and a rasagiline(RAS)group,with 6 mice in each group.Except for the mice in the NC group,all mice were injected intraperitoneally with MPTP[30 mg/(kg·bw)]to establish a PD mouse model.After the models were successfully established,mice in the ACU group received acupuncture at Baihui(GV20)and bilateral Yanglingquan(GB34)for 15 min,once a day for 14 consecutive days.Mice in the RAS group were treated with gavage of rasagiline mesylate[0.5 mg/(kg·bw)],once daily for 14 d.Mouse balance and motor functions were detected using the mouse fatigue rotating rod apparatus.Immunohistochemistry staining was used to detect the number of tyrosine hydroxylase(TH)-positive neurons and the protein expression levels of special AT-rich sequence-binding protein 1(SATB1),p21,and p53 in the substantia nigra(SN)region of the mouse brain in each group.The glutathione peroxidase(GSH-Px)activity of mouse brain SN tissue was detected by enzyme-linked immunosorbent assay.The protein expression levels of interleukin(IL)-6 and senescence-associatedβ-galactosidase(SA-β-gal)in the SN tissue of mice in each group were detected by Western blotting.The relative expression of SATB1,p21,and p53 mRNA in the SN of each group was detected by real-time quantitative polymerase chain reaction.Results:Compared to the NC group,the overall rod performance(ORP)score,the number of TH-positive neurons,and GSH-Px activity in the SN region were significantly lower in the mice in the MPTP group(P<0.01);compared to the MPTP group,the ORP score,the number of TH-positive neurons,and GSH-Px activity were significantly increased in the ACU group and the RAS group(P<0.01 or P<0.05).Compared to the N目的:观察针刺对帕金森病(PD)模型小鼠运动功能的影响,并从细胞衰老角度探究针刺对PD的神经保护作用。方法:将C57BL/6J小鼠随机分为正常(NC)组、1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)组、针刺(ACU)组和雷沙吉兰(RAS)组,每组6只。除正常组小鼠外,其余各组小鼠腹腔注射MPTP[30 mg/(kg·bw)]构建PD小鼠模型。ACU组小鼠予针刺百会和双侧阳陵泉,每次15 min,每日1次,连续治疗14 d。RAS组小鼠采用甲磺酸雷沙吉兰[0.5 mg/(kg·bw)]进行灌胃治疗,每日1次,连续治疗14 d。采用疲劳转棒仪检测小鼠平衡和运动能力。采用免疫组织化学染色法检测各组小鼠脑黑质部位TH阳性神经元数量和SATB1、p21、p53蛋白表达水平。采用酶联免疫吸附法检测小鼠中脑黑质组织谷胱甘肽过氧化物酶(GSH-Px)活力。采用免疫印迹法检测各组小鼠脑黑质组织白细胞介素(IL)-6、衰老相关β-半乳糖苷酶(SA-β-gal)蛋白表达水平。采用实时荧光定量逆转录聚合酶链反应检测各组小鼠中脑黑质组织AT富集序列特异性结合蛋白1(SATB1)、p21、p53 mRNA相对表达量。结果:与NC组相比,MPTP组小鼠转棒实验(ORP)评分、中脑黑质区域TH阳性神经元数量和GSH-Px活性显著降低(P<0.01);与MPTP组相比,ACU组和RAS组ORP评分、TH阳性神经元数量和GSH-Px活性显著增加(P<0.01或P<0.05)。与NC组相比,MPTP组小鼠中脑黑质IL-6和SA-β-gal蛋白、p21与p53的蛋白及mRNA表达水平显著增加(P<0.01);与MPTP组相比,ACU组和RAS组小鼠中脑黑质IL-6和SA-β-gal蛋白、p21与p53的蛋白及mRNA表达水平显著降低(P<0.01或P<0.05)。与NC组相比,MPTP组小鼠中脑黑质SATB1蛋白和mRNA相对表达量显著降低(P<0.01);与MPTP组小鼠相比,ACU组和RAS组小鼠SATB1蛋白和mRNA相对表达量显著增加(P<0.01或P<0.05)。结论:针刺可以改善PD模型小鼠运动功能,增加黑质部位TH阳性神经元数量。针刺对PD小鼠的神经保护作用可能与调节SATB1

关 键 词：Acupuncture Therapy Point Baihui(GV20) Point Yanglingquan(GB34) Parkinson Disease Signal Transduction Cell Aging Mice 

分 类 号：R2-03[医药卫生—中医学]