Effects of different acupuncture and moxibustion methods on knee joint cartilage morphology,serum inflammatory factors,and intestinal flora in rats with knee osteoarthritis  

不同刺灸法对膝骨关节炎大鼠膝关节软骨形态、血清炎症因子及肠道菌群的影响

作　　者：DONG Qi JIA Yejuan QIN Lingling ZHANG Xiaoqin XU Jing LI Xiaofeng 董琪;贾叶娟;秦玲玲;张小琴;徐晶;李晓峰(School of Acupuncture-moxibustion and Tuina,Hebei University of Chinese Medicine,Shijiazhuang 050200,China)

机构地区：[1]School of Acupuncture-moxibustion and Tuina,Hebei University of Chinese Medicine,Shijiazhuang 050200,China

出　　处：《Journal of Acupuncture and Tuina Science》2025年第1期22-35,共14页针灸推拿医学(英文版)

基　　金：国家自然科学基金资助项目,No.82004449;河北省中医药管理局中医药类科研计划项目,No.2020133。

摘　　要：Objective:To compare the effects of manual acupuncture(MA),electroacupuncture(EA),and moxibustion on knee joint cartilage morphology,serum inflammatory factors interleukin(IL)-6 and IL-10,matrix metalloproteinase 13(MMP13),and intestinal flora composition in knee osteoarthritis(KOA)model rats.Methods:Forty male specific-pathogen-free Sprague-Dawley rats aged seven weeks were randomly divided into a normal group(n=8)and a KOA modeling group(n=32).The KOA model was established using sodium iodoacetate induction.The KOA modeling rats were further randomly divided into a model group,an MA group,an EA group,and a moxibustion group,with 8 rats in each group.In the MA,EA,and moxibustion groups,interventions targeting the right Futu(ST32)and Zusanli(ST36)were performed for 15 min,once every other day,for 14 sessions.The normal and model groups were bundled on the self-made fixation frame for 15 min.The rat knee joint diameter was measured on the 8th day of adaptive feeding,after successful modeling,and after the 14th intervention.Lequesne behavioral scoring was performed after successful modeling and after the 14th intervention.After the 14th intervention,hematoxylin-eosin(HE)staining and Masson staining were performed with the cartilage sections of the right knee joint.The pathomorphological changes of the rat joint cartilage were observed and quantified by Mankin’s score.Enzyme-linked immunosorbent assay was used to detect the rat serum levels of IL-6,IL-10,and MMP13.Additionally,16S rDNA sequencing was used to detect the composition of rat fecal flora.Results:Compared to the normal group,the right knee joint diameter and the Lequesne score were significantly increased in the model group(P<0.01).Compared to the model group,the right knee joint diameter and the Lequesne score of rats in the MA,EA,and moxibustion groups were significantly reduced(P<0.01),with no significant differences among the three intervention groups(P>0.05).HE staining and Masson staining revealed disordered cartilage structure in the model group,目的:比较手针、电针、艾灸3种刺灸法对膝骨关节炎(KOA)模型大鼠膝关节软骨形态、血清炎症因子白介素(IL)-6和IL-10、基质金属蛋白酶13(MMP13)及肠道菌群组成的效应差异。方法:七周龄雄性清洁级Sprague-Dawley大鼠40只,随机选取8只作为正常组,其余32只采用碘乙酸钠诱导法复制KOA模型。模型复制成功后,随机分为模型组、手针组、电针组和艾灸组,每组8只。手针组、电针组和艾灸组均取右侧伏兔、足三里进行干预,每次15 min,隔日干预一次,共14次。正常组和模型组固定于自制固定架15 min。于适应性喂养第8天、造模成功后、第14次干预后分别测量大鼠膝关节直径。于造模成功后和第14次干预后进行Lequesne行为学评分。于第14次干预后,对右膝关节软骨切片进行苏木素-伊红(HE)染色及Masson染色,观察大鼠关节软骨病理形态学改变,并通过Mankin’s评分量化评估;酶联免疫吸附试验检测大鼠血清中IL-6、IL-10及MMP13含量;16S rDNA测序分析检测大鼠粪便菌群组成。结果:与正常组相比,模型组大鼠右膝关节直径显著增加(P<0.01),Lequesne评分显著升高(P<0.01)。与模型组相比,手针组、电针组和艾灸组大鼠右膝关节直径均显著降低(P<0.01),Lequesne评分显著降低(P<0.01);三个干预组之间差异无统计学意义(P>0.05)。关节软骨病理切片HE染色及Masson染色显示模型组膝关节软骨组织形态结构紊乱,干预后手针组、电针组和艾灸组的膝关节软骨组织形态结构均得到改善。Mankin’s评分结果显示,与正常组相比,模型组评分显著增高(P<0.05);与模型组相比,手针组、电针组和艾灸组的评分均显著降低(P<0.05)。与正常组相比,模型组血清IL-6及MMP13含量升高(P<0.05),IL-10含量降低(P<0.05)。与模型组相比,手针组、电针组和艾灸组血清IL-6含量均显著降低(P<0.05),IL-10均显著升高(P<0.05),但三组间无统计学差异(P>0.05);艾灸组

关 键 词：Acupuncture Therapy Electroacupuncture Therapy Moxibustion Therapy Osteoarthritis Knee INTERLEUKINS Gastrointestinal Microbiome RATS 

分 类 号：R2-03[医药卫生—中医学]