鸡血藤醇提取物对玉米赤霉烯酮导致KK-1细胞损伤的改善作用  

Improvement Effect of Ethanol Extracts of Spatholobus suberctus Dunn on Zearalenone-Induced KK-Cell Damage

作　　者：何扬 刘惠心 谢宗固 司红彬[1] HE Yang;LIU Huixin;XIE Zonggu;SI Hongbin(Guangxi Key Laboratory of Animal Breeding,Disease Control and Prevention,College of Animal Science and Technology,Guangxi University,Guangxi Grass Station,Guangxi Nanning 530000,China)

机构地区：[1]广西大学动物科学技术学院,广西畜禽繁育与疾病防控重点实验室,广西壮族自治区牧草工作站,广西南宁530000

出　　处：《饲料工业》2025年第3期118-124,共7页Feed Industry

基　　金：国家现代农业产业技术体系广西家禽产业创新团队项目[nycytxgxcxtd-2024-19-2];国家自然科学基金联合基金项目[U22A20523];中央引导地方专项[桂科计字〔2023〕55号];广西大学自科创新倍增计划项目[2024BZPT020]。

摘　　要：研究旨在探讨鸡血藤醇提取物对玉米赤霉烯酮(ZEA)诱导的小鼠卵巢颗粒细胞(KK-1)的影响。选用KK-1细胞作为研究对象,用不同浓度ZEA处理,观察细胞的生长情况,ELISA检测孕酮(PG)和雌二醇(E2)含量,分析细胞凋亡情况;使用半定量RT-qPCR在安全浓度范围下检测鸡血藤醇提取物对ZEA诱导的KK-1细胞中雌激素受体ERα和ERβmRNA水平的影响。结果表明:ZEA在20μmol/L 24 h和10μmol/L 48 h条件下导致KK-1细胞50%死亡。添加20、10μg鸡血藤醇提取物使ZEA诱导的KK-1细胞增殖和凋亡水平改善,同时使E2和PG的分泌功能得以恢复;此外,鸡血藤醇提取物还影响ERα和ERβmRNA水平。综上所述,鸡血藤醇提取物能够促进KK-1细胞的生长和PG、E2激素的分泌功能,增强细胞抗凋亡能力,并且调节ERα和ERβmRNA水平,进而对ZEA诱导的KK-1损伤起到保护作用。This study aimed to investigate the effects and potential mechanisms of ethanol extracts of Spatholobus suberctus Dunn on zearalenone(ZEA)-induced damage in ovarian granulosa cells(KK-1 cells).KK-1 cells were selected as the research object,treated with different concentrations of ZEA.And the apoptosis was analyzed by observation of cell growth and detection of progesterone(PG)and estradiol(E2)levels(ELISA)and RT-qPCR to detect the effect of ethanol extracts of Spatholobus suberctus Dunn on the gene expression levels of estrogen receptor ERαand ERβmRNA in ZEA-induced KK-1 cells.The results showed that ZEA caused 50%cell death in KK-1 cells at concentrations of 20μmol/L for 24 h and 10μmol/L for 48 h.Conversely,ZEA-induced damage on KK-1 cells were improved by ethanol extracts of Spatholobus suberctus Dunn at concentrations of 20μg and 10μg,while restoring the secretion function of E2 and PG.In addition,its pharmacological effects were exerted by affecting the expression levels of ERαand ERβmRNA.In conclusion,this study suggests that the growth of KK-1 cells and the secretion function of PG and E2 hormones can be promoted by enhancing their anti-apoptotic ability,and regulate the expression levels of ERαand ERβ,thereby exerting a protective effect on ZEA-induced damage on KK-1 cells.

关 键 词：鸡血藤醇提取物 玉米赤霉烯酮 小鼠卵巢颗粒细胞 细胞增殖和凋亡 雌激素受体 

分 类 号：S813[农业科学—畜牧学]