Ce-UiO-66 nanozymes with charge-regulated uricase-like activity for enzyme/reagent-free detection of uric acid  

作　　者：Zhixiong Guo Da Chen Wei Gao Yongquan Qu Zhimin Tian 

机构地区：[1]Key Laboratory of Special Functional and Smart Polymer Materials of Ministry of Industry and Information Technology,School of Chemistry and Chemical Engineering,Northwestern Polytechnical University,Xi’an 710054,China [2]State Key Laboratory of Solidification Processing,Center for Nano Energy Materials,School of Materials Science and Engineering,Northwestern Polytechnical University,Xi’an 710072,China

出　　处：《Nano Research》2025年第2期675-686,共12页纳米研究(英文版)

基　　金：This work was supported by the National Key Research and Development Program of China(No.2021YFB3500904);the National Natural Science Foundation of China(No.22475173);the Key Research and Development Program of Shaanxi(No.2024SF-YBXM-439);Authors also acknowledge the support from Fundamental Research Funds for the Central Universities(No.D5000210635).

摘　　要：Designing highly performed uricase-like nanozymes through enzymatic-like colorimetric analysis system is of vital significance for the quantitative detection of uric acid(UA).Herein,series of Ce-UiO-66 nanozymes with different surface charges through the ligand engineering strategy were rationally designed and synthesized as efficient uricase mimics with tailorable uricase-like activities to catalytically convert UA into allantoin and H2O2.Importantly,by tuning the functional groups of 1,4-benzoic acid ligands,we explored the relationships between the surface charges of Ce-UiO-66-X(X=H,NO_(2),Br,CH_(3),and OH)nanozymes and their uricase-like activity.Among them,Ce UiO-66-CH_(3) with the moderate surface charge exhibited optimal substrate adsorption and product desorption,displaying the highest uricase-like activity.Significantly,H2O2,as the product of UA oxidation,enabled Ce-UiO-66-CH_(3) itself as a dose-dependent chromogenic substrate of H_(2)O_(2),giving a white-to-orange color evolution due to the Ce-UiO-66-CH_(3)-to-CeO_(2) phase transition.Afterwards,a smartphone-assisted all-in-one enzyme/reagent-free biosensor based on Ce-UiO-66-CH_(3) was established for the precise visual detection of UA analysis,which was featured by a wide detection range(31–4000µM),a high sensitivity(limit of detection:8.9µM),a rapid response(-3 min),a high structural stability,and a high anti-interference ability.

关 键 词：Ce-UiO-66 uricase-like nanozyme colorimetric uric acid analysis enzyme/reagent-free detection smartphone 

分 类 号：O62[理学—有机化学]