赤芍、大血藤延缓干燥综合征唾液腺损伤的疗效及机制研究  

Therapeutic Effect and Mechanism of Paeoniae Radix Rubra and Sargentodoxae Caulis in Delaying Salivary Gland Injury in Sjogren's Syndrome

作　　者：刘瑞华[1] 唐晓颇[1] 郭子琳 杨可非 刘文靖 周新尧[1] 姜泉[1] LIU Ruihua;TANG Xiaopo;GUO Zilin;YANG Kefei;LIU Wenjing;ZHOU Xinyao;JIANG Quan(Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)

机构地区：[1]中国中医科学院广安门医院,北京100053

出　　处：《中国中医基础医学杂志》2025年第2期252-258,共7页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：中国中医科学院科技创新工程项目(CI2021A01510、CI2021A01502);中央级公益性科研院所基本科研业务费专项(ZZ15-XY-PT-11,ZZ15-YQ-023);国家自然科学基金面上项目(82374285)。

摘　　要：目的观察赤芍、大血藤对干燥综合征(Sjogren's syndrome,SS)唾液腺损伤的治疗作用,并探讨其可能通过白细胞介素(IL)-2调节滤泡辅助性T细胞(Tfh)/调节性T细胞(Treg)失衡延缓腺体损伤的生物作用机制。方法雌性NOD小鼠随机分为5组,即模型组、赤芍组、大血藤组、芍藤组、IL-2组,每组10只。10只雌性BALB/c小鼠作为正常组。9周龄开始干预,赤芍组、大血藤组、芍藤组分别予赤芍水煎液、大血藤水煎液、芍藤水煎液灌胃,每日1次;IL-2组予注射用重组人IL-2皮下注射,隔日1次,各组干预共3周。干预期间观测各组小鼠一般情况、饮水量、唾液流率。干预结束后采用HE染色法评估颌下腺病理改变、采用免疫组化方法检测颌下腺IL-2表达情况,采用流式细胞术检测脾脏Tfh、Treg细胞水平。结果与正常组比较,模型组饮水量明显增多(P<0.05),唾液流率明显降低(P<0.05),颌下腺病理评分明显增高(P<0.05),颌下腺IL-2表达明显降低(P<0.05),脾脏Tfh/Treg细胞比例显著升高(P<0.05);与模型组比较,赤芍组、大血藤组、芍藤组、IL-2组的饮水量均明显降低(P<0.05),唾液流率明显增多(P<0.05),颌下腺病理评分下降(P<0.05),IL-2的表达均明显升高(P<0.05),Tfh/Treg细胞比例均有明显下调(P<0.05),各治疗组比较差异无统计学意义(P>0.05)。结论赤芍、大血藤能够减轻NOD小鼠颌下腺淋巴细胞浸润,改善腺体结构和功能,增加唾液分泌。赤芍、大血藤可能通过调节IL-2水平恢复Tfh/Treg稳态从而发挥其治疗SS的作用。Objective To observe the therapeutic effects of Paeoniae Radix Rubra and Sargentodoxae Caulis on salivary gland injury in Sjogren's syndrome(SS),and to explore the biological mechanism of delaying the gland injury by regulating the imbalance of Follicular helper T cell(Tfh)and Regulatory cell(Treg)through interleukin(IL)-2.Methods Female NOD mice were randomly divided into 5 groups:model group,Paeoniae Radix Rubra group,Sargentodoxae Caulis group,Paeoniae Radix Rubra and Sargentodoxae Caulis group,and IL-2 group,with 10 mice in each group.10 female BALB/c mice were used as the normal group.Intervention was initiated at the age of 9 weeks,and Paeoniae Radix Rubra group,Sargentodoxae Caulis group,Paeoniae Radix Rubra and Sargentodoxae Caulis group were respectively given the decoction of Paeoniae Radix Rubra,Sargentodoxae Caulis,Paeoniae Radix Rubra and Sargentodoxae Caulis by gastric gavage once a day,and IL-2 group was given the recombinant human IL-2 by subcutaneous injection once every other day,and normal group and model group were given physiological saline by gavage once a day.Each group was intervened for a total of 3 weeks.During the intervention period,observe the general situation,water intake,and the saliva flow of each group of mice.After the intervention,HE staining was used to evaluate the pathological changes in the submandibular gland,immunohistochemistry was used to detect the expression of IL-2 in the submandibular gland,and flow cytometry was used to detect the levels of Tfh and Treg cells in the spleen.Results Compared with the normal group,the model group had significantly higher water intake(P<0.05),lower salivary flow rate(P<0.05),increased pathological grade of the submandibular gland(P<0.05),lower expression of IL-2 in the submandibular gland,elevated ratio of splenic Tfh/Treg cells.Compared with model group,the Paeoniae Radix Rubra group,Sargentodoxae Caulis group,Paeoniae Radix Rubra and Sargentodoxae Caulis group,and IL-2 group has significantly lower water intake(P<0.05),higher salivar

关 键 词：干燥综合征 唾液腺损伤 赤芍 大血藤 Tfh/Treg平衡 IL-2 

分 类 号：R285[医药卫生—中药学]