特异性检测Cys荧光探针的合成及性质研究  

Synthesis and Properties of Fluorescent Probe for Specific Detection of Cys

作　　者：朱霜霜 汪旺斐 陈娟杰 冯燕 李莉 黄森 谷晓霞 吕帆 ZHU Shuangshuang;WANG Wangfei;CHEN Juanjie;FENG Yan;LI Li;HUANG Sen;GU Xiaoxia;LV Fan(Department of Chemistry,Wannan Medical College,Anhui Wuhu 241002,China)

机构地区：[1]皖南医学院药学院化学教研室,安徽芜湖241002

出　　处：《广州化工》2025年第3期42-45,53,共5页GuangZhou Chemical Industry

基　　金：安徽省高等学校科学研究项目(KJ2021A0847,2024AH051920);安徽省大学生创新训练项目(S202210368063);国家级大学生创新训练项目(202210368036);校中青年科研基金项目(WK2024ZQNZ11)。

摘　　要：半胱氨酸(Cysteine,Cys)作为生物体内一种重要的含巯基氨基酸,参与众多生理功能和病理生理过程。因此,开发一种能够选择性识别半胱氨酸的荧光探针具有重要的研究意义。在本研究中,我们设计了一种新型的比率荧光探针Probe-S,它含有氟硼二吡咯(BODIPY)荧光团和响应基团对甲氧基苯硫醚。在PBS buffer∶DMSO=5∶1,pH=7.3中,500 nm激发波长条件下,向Probe-S(10 mM)中加入0~100 mM的Cys,溶液立即由暗红色变为绿色,同时伴随着明显的荧光增强现象。结果表明探针Probe-S对Cys具有良好的灵敏度和选择性,有望成为一种监测半胱氨酸在生理或病理过程中的新工具。Cysteine(Cys),a crucial sulfhydryl amino acid,plays a pivotal role in numerous physiological functions and pathophysiological processes.Hence,it is of paramount importance to develop a fluorescent probe capable of selectively recognizing cysteine.In this study,we have devised an innovative ratiometric fluorescent probe Probe-S,which contained the boron dipyrromethene(BODIPY)fluorophore and response group p-methoxyphenthioether.Under the conditions of PBS buffer∶DMSO=5∶1,pH=7.3,with an excitation wavelength of 500 nm,the addition of Cys ranging from 0~100 mM to Probe-S(10 mM)resulted in an immediate transformation from dark red to brilliant green light solution along with conspicuous fluorescence enhancement.These findings demonstrate that Probe-S exhibits excellent sensitivity and selectivity towards Cys and holds promise as a novel tool for monitoring cysteine levels during physiological and pathological processes.

关 键 词：半胱氨酸(Cys) 氟硼二吡咯(BODIPY) 荧光探针 选择性识别 

分 类 号：O621.2[理学—有机化学]