LncRNA SNHG1调节鲍曼不动杆菌感染肺上皮细胞  

Role Of LncRNA SNHG1 in Acinetobacter baumannii Infection of Lung Epithelial Cells

作　　者：高丽丽 秦廷洋 龙红 马晓春 GAO Lili;QIN Tingyang;LONG Hong;MA Xiaochun(Qingdao Agricultural University,Qingdao 266109,China;Zunyi Medical University,Zunyi 563000,China;Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China)

机构地区：[1]青岛农业大学,青岛266109 [2]遵义医科大学,遵义563000 [3]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国动物传染病学报》2024年第6期1-7,共7页Chinese Journal of Animal Infectious Diseases

基　　金：青岛农业大学博士基金项目(663-1122005);贵州省科技厅基础研究计划(黔科合基础[2020]1Y350)。

摘　　要：研究表明,lncRNA SNHG1的异常表达与炎症相关疾病有关。为了解SNHG1在鲍曼不动杆菌感染肺部过程中的作用,本研究采用鲍曼不动杆菌感染人肺上皮细胞建立细胞感染模型,发现SNHG1在鲍曼不动杆菌感染肺上皮细胞后表达上调。为进一步探讨SNHG1对于感染过程的调控作用,我们构建了SNHG1的过表达载体,并合成其干扰RNA,通过过表达和干扰lncRNA SNHG1研究鲍曼不动杆菌感染后SNHG1对抗菌肽hBD-2和细胞因子IL-6及IL-8表达的影响。结果显示,鲍曼不动杆菌感染A549细胞后,hBD-2、IL-6和IL-8表达显著上升,表明感染模型构建成功;当SNHG1在A549细胞中过表达后,IL-6和IL-8转录水平上升,上清液中hBD-2的分泌量增加。当干扰SNHG1表达后,hBD-2的分泌量略有下降,差异不显著。综上,lncRNA SNHG1通过调节抗菌肽hBD-2及细胞因子IL-6、IL-8参与鲍曼不动杆菌感染A549过程。Several studies have shown that aberrant expression of lncRNA SNHG1 is associated with inflammation-related diseases.To understand the role of SNHG1 in lung infection,Acinetobacter baumannii was used to infect human lung epithelial cells to establish a cellular infection model in this study.To further investigate the regulatory role of SNHG1 in the infection process,we constructed an overexpression vector of SNHG1 and synthesized its interfering RNA to investigate the effect of SNHG1 on the expression of antimicrobial peptide hBD-2 and cytokines IL-6 and IL-8.The results showed that the expression of hBD-2,IL-6 and IL-8 significantly increased after A549 cells were infected with A.baumannii,indicating that the infection model was successfully constructed.When SNHG1 was overexpressed in A549 cells,the transcript levels of IL-6 and IL-8 increased and the secretion of hBD-2 in the supernatant also increased.When SNHG1 was interfered,hBD-2 was slightly decreased.In conclusion,lncRNA SNHG1 was involved in the infection process through regulation of antimicrobial peptide hBD-2 and cytokines IL-6 and IL-8.

关 键 词：鲍曼不动杆菌 肺上皮细胞 LncRNA-SNHG1 HBD-2 IL-6 

分 类 号：R563.1[医药卫生—呼吸系统]