蚂蟥水肿病病原的分离鉴定与药敏分析  

Isolation,Identification and Drug Susceptibility of the Etiological Agent of Lethal Edema of Whitmania pigra

作　　者：王海华[1] 付辉云[1] 王帅兵[2] 马本贺 熊良伟[2] 李涵 王梦杰 李燕华[1] WANG Haihua;FU Huiyun;WANG Shuaibing;MA Benhe;XIONG Liangwei;LI Han;WANG Mengjie;LI Yanhua(Nanchang Key Laboratory of Special Aquactic Breeding and Healthy Aquaculture,Jiangxi Fisheries Research Institute,Nanchang 330039,China;Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China)

机构地区：[1]江西省水产科学研究所,南昌市特种水产繁育与健康养殖重点实验室,南昌330039 [2]江苏农牧科技职业学院,泰州225300

出　　处：《中国动物传染病学报》2024年第6期36-41,共6页Chinese Journal of Animal Infectious Diseases

基　　金：江西省重点研发计划项目(20212BBG73040);泰州市科技支撑计划项目(TS20)。

摘　　要：为探究蚂蟥水肿病的病原及其敏感药物,筛选出有效防控蚂蟥水肿病药物,采用稀释平板分离法从蚂蟥病灶分离得到病原菌株GC21071301;采用病理特征观察、回归感染、生理生化特性测定和16S rDNA分子生物学鉴定等方法对病原菌株进行种类鉴定,并通过药敏试验筛选有效治疗药物。研究结果显示:蚂蟥水肿病的临床病理特征为躯体水肿、发白,严重呈透明状,体腔有大量积液,病蛭末端或中间有硬节,逐渐变坏死、僵硬并蔓延至整个躯体,病死率较高;健康蚂蟥注射菌株GC21071301回归感染后24 h内即开始出现死亡,于第5 d全部死亡,症状主要表现与水肿病一致;蚂蟥水肿病的病原菌株GC21071301为维氏气单胞菌(Aeromonas veronii),四环素类、喹诺酮类和氨基糖苷类抗生素是蚂蟥水肿病的有效治疗药物。本研究结果可为蚂蟥水肿病诊断和防治提供参考。To investigate the etiological agent and drug susceptibility associated with lethal edema disease of Whitmania pigra,an Aeromonas veronii strain GC21071301 was isolated from lesions of a diseased leech using a dilution-plate method.The strain GC21071301 was identified as Aeromonas veronii by pathological observation,regression infection,determination of physiological and biochemical characteristics and 16S rDNA molecular biological analysis.The results showed that the clinicopathological characteristics of lethal edema of Whitmania pigra included edema,whiteness,severe transparency,a large amount of fluid accumulation in the body cavities and hard nodes at the end or middle of the body.These pathological changes gradually became necrosis,stiffness and spread to the whole body with high fatality.The healthy leeches were experimentally infected with the strain GC21071301.The infected leeches started to die within 24 h post infection and all died on day 5.The major signs were consistent with lethal edema of Whitmania pigra.Moreover,29 antibacterial drugs were tested for their susceptibility,of which tetracycline,quinolones and aminoglycoside antibiotics were effective drugs for the treatment of lethal edema of Whitmania pigra.The results of this research could provide a reference for lethal edema disease in Whitmania pigra.

关 键 词：蚂蟥 水肿病 维氏气单胞菌 分离鉴定 药敏试验 

分 类 号：S858.94[农业科学—临床兽医学]