鸡包涵体肝炎鉴别诊断方法的建立及其应用  

Development and Application of Differential Diagnosis for Chicken Inclusion Body Hepatitis

作　　者：路晓 韩易航 张帆 赵凌云 刘丽萍 宋敏训[1] 于可响[1] LU Xiao;HANG Yihang;ZHANG Fan;ZHAO Lingyun;LIU Lipin;SONG Minxun;YU Kexiang(Institute of Poultry,Shandong Academy of Agricultural Sciences,Shandong provincial Key Laboratory of Poultry Disease Diagnosis and Immunology,Jinan 250023,China;College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;Yantai Aishijin Animal Health Products Co.,Ltd,Yantai 264000,China;Yantai Zhongchong Food Co.,Ltd.,Yantai 264000,China)

机构地区：[1]山东省农业科学院家禽研究所,山东省家禽疫病诊断与免疫重点实验室,济南250023 [2]内蒙古农业大学兽医学院,呼和浩特010018 [3]烟台爱士津动物保健品有限公司,烟台264000 [4]烟台中宠食品股份有限公司,烟台264000

出　　处：《中国动物传染病学报》2024年第6期69-74,共6页Chinese Journal of Animal Infectious Diseases

基　　金：山东省重点研发计划(2019GNC106044);山东省现代农业产业技术体系家禽创新团队计划(SDAIT-11-01);山东省农业科学院创新工程项目(CXGC2021A12、CXGC2021A49);烟台市科技创新发展计划(2021NYNC021)。

摘　　要：鸡包涵体肝炎主要由Ⅰ群禽腺病毒血清8a型(FAdV-8a)、8b型(FAdV-8b)和11型(FAdV-11)病毒引起,这3种血清型在临床上无法区分,因此有必要建立一种鉴别诊断方法。根据这3种血清型病毒的基因组设计了3对特异性引物,将这些引物放在同一反应体系中,通过优化条件建立了多重PCR鉴别方法。结果显示,该方法具有良好的特异性、敏感性和重复性,能快速区分鸡包涵体肝炎中血清8a型、8b型和11型病毒的感染或是上述病毒的混合感染。该方法在临床上进行了初步应用,与经典的病毒分离方法相比,FAdV-8a、FAdV-8b和FAdV-11的符合率分别为100%、98%和98%。Chicken inclusion body hepatitis is mainly caused by group I avian adenovirus serotypes 8a(FAdV-8a),8b(FAdV-8b)and 11(FAdV-11).These three serotypes cannot be distinguished clinically,so it is necessary to develop a differential diagnostic method.Three pairs of specific primers were designed according to the genomes of these three viruses.These primers were placed in the same reaction system and a multiplex PCR method was developed followed by optimization.The results showed that the method had good specificity,sensitivity and repeatability and quickly distinguish whether chicken inclusion body hepatitis was caused by single infection or mixed infection of these three viruses.Compared with the classical virus isolation method,the coincidence rates of the multiplex PCR developed here were 100%,98%and 98%for FAdV-8a,FAdV-8b and FAdV-11,respectively.

关 键 词：鸡包涵体肝炎 多重PCR 鉴别诊断 

分 类 号：S858.31[农业科学—临床兽医学]