1株流产布鲁氏菌脂多糖单克隆抗体的制备及其特性分析  

Preparation and Characterization of a Monoclonal Antibody Against Lipopolysaccharide of Brucella abortus

作　　者：瞿晶 刘佳萌[1] 田明星 王少辉[1] 祁晶晶[1] 于圣青[1] QU Jing;LIU Jiameng;TIAN Mingxing;WANG Shaohui;QI Jingjing;YU Shengqing(Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;Songjiang District Center for Animal Disease Control and Prevention,Shanghai 201699,China)

机构地区：[1]中国农业科学院上海兽医研究所,上海200241 [2]上海市松江区动物疫病预防控制中心,上海201699

出　　处：《中国动物传染病学报》2024年第6期128-134,共7页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金(32072828,31972723);中国农业科学院科技创新工程(SHVRI-ASTIP-2014-8)。

摘　　要：布鲁氏菌是一种兼性胞内寄生菌,感染人和动物,引起严重的人畜共患传染病。脂多糖(LPS)是布鲁氏菌主要免疫原性组分,其在布鲁氏菌病血清学诊断中起到关键作用。本研究通过纯化的LPS作为包被抗原,筛选鉴定到1株分泌针对流产布鲁氏菌LPS的单克隆抗体的杂交瘤细胞株51C。细胞分泌抗体稳定性试验发现,细胞盲传30代和液氮冻存12周后,不影响51C分泌单克隆抗体的能力;单克隆抗体亚型分析发现,51C分泌的抗体亚类为IgG3,轻链为κ链;免疫印迹试验分析发现,单克隆抗体可以与流产布鲁氏菌和猪种布鲁氏菌LPS反应,不能与马耳他布鲁氏菌和其他细菌的LPS进行反应。本研究制备的单克隆抗体为布鲁氏菌病的诊断和细菌分型奠定基础。Brucella is a facultative intracellular bacterium that infects human and animals,causing serious zoonotic infectious disease.Lipopolysaccharide(LPS)is the main immunogenic component of Brucella,which plays an important role in serological diagnosis of brucellosis.In this study,a hybridoma cell line 51C secreting monoclonal antibody(mAb)was obtained by screening in ELISA using the purified LPS as the coating antigen.The cell line 51C stably secreted the mAb for blind 30 passages and storage in liquid nitrogen for 12 weeks.The mAb 51C was subtyped as IgG3 withκlight chain.Western blot showed that the mAb 51C reacted with the LPS of B.abortus and B.suis but not with the LPS from B.melitensis and other bacteria.The preparation of the mAb 51C in this study laid a foundation for diagnosis of brucellosis and bacterial typing.

关 键 词：布鲁氏菌 脂多糖 单克隆抗体 特性 

分 类 号：S859.797[农业科学—临床兽医学]