2021年华东地区鸡传染性支气管炎病毒分离、鉴定及S1基因遗传特征分析  

Isolation of Avian Infectious Bronchitis Virus and Genetic Characterization of S1 Gene from East China in 2021

作　　者：蓝胜芝 侯博[2] 邵国青 王晨燕[2] LAN Shengzhi;HOU Bo;SHAO Guoqing;WANG Chenyan(Zhejiang Ceva Ebvac Co.,Ltd.,Hangzhou 310018,China;Fujian Animal Disease Control Technology Development Center,Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;Institute of Veterinary Medicine,Jiangsu Academyof Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]浙江诗华诺倍威生物技术有限公司,杭州310018 [2]福建省农业科学院畜牧兽医研究所,福建省畜禽疫病疾病防治工程技术研究中心,福州350013 [3]江苏省农业科学院兽医研究所,南京210014

出　　处：《中国动物传染病学报》2024年第6期169-175,共7页Chinese Journal of Animal Infectious Diseases

基　　金：福建省农业科学院科技创新团队(CXTD2021014-3);福建省农业科学院“5511”协同创新重点项目(XTCXGC2021008)。

摘　　要：本研究在2021年从华东地区肉鸡或蛋鸡群中采集了187份具有呼吸道症状的患病鸡气管、肺脏和肾脏混合样本,通过鸡胚分离、RT-PCR和基因测序等方法分离鉴定出IBV,并对其S1基因测序,通过构建系统进化树进行遗传进化分析。结果显示:187份临床病料中检测出79份阳性样品,将其接种SPF鸡胚共分离鉴定出39株IBV,S1进化树分析结果表明主要流行的基因型为GⅠ-19、GⅠ-13、tl/CH/LDT3/03型和GⅥ,其中GⅠ-19为优势基因型,其次是GⅠ-13。本研究初步明确了华东地区2021年IBV分离株的遗传进化特点,对IBV临床防控具有重要的参考意义。In this study,we collected 187 samples from tracheas,lungs and kidneys of broilers and layer flocks suspected to be infected with Infectious bronchitis virus(IBV)in 2021 in East China for isolation of IBV by inoculating SPF chicken embryos.The isolated IBVs were identified by RT-PCR and sequencing followed by phylogenetic tree analysis based on S1 gene.The results showed that 39 IBV strains were isolated from 79 positive clinical samples using SPF chicken embryos.Phylogenetic analysis revealed that the genotypes of these isolates included GI-19,GI-13,tl/CH/LDT3/03 type and GVI with GI-19 being the most predominant genotype,and GI-13 was the second most dominant genotype.The genetic evolution characteristics of these isolates in East China had important significance for clinical prevention and control of IBV.

关 键 词：传染性支气管炎病毒(IBV) 分离 S1基因 进化树分析 

分 类 号：S858.31[农业科学—临床兽医学]