富血小板血浆结合周期性机械牵拉对C2C12肌管萎缩的影响及机制探讨  

Effects and mechanism of platelet-rich plasma combined with periodic mechanical stretching on C2C12 myotube atrophy

作　　者：谢晓婷 何永 马彦韬 王博 贾佩玉 郑拥军 XIE Xiaoting;HE Yong;MA Yantao(Department of Pain,Huadong Hospital,Shanghai Key Laboratory of Clinical Geriatric Medicine,Shanghai,200040)

机构地区：[1]复旦大学附属华东医院疼痛科上海市老年医学临床重点实验室,上海市200040 [2]上海体育大学运动健康学院

出　　处：《中国康复医学杂志》2025年第1期8-14,共7页Chinese Journal of Rehabilitation Medicine

基　　金：国家科委临床医学研究资助项目(21MC1930200);国家重点研发计划专项项目(2017YFB0403803)。

摘　　要：目的:研究富血小板血浆(platelet-rich plasma,PRP)结合周期性机械牵拉对C2C12肌管萎缩的影响及机制。方法:通过蛋白质印记(Western Blot,WB)检测MyoD、MyoG的表达选取最适PRP浓度。随后探究PRP结合周期性机械牵拉(Str)对C2C12细胞的促分化作用;用TNF-α诱导C2C12肌管萎缩,改良吉姆萨染色验证模型;为探究2%PRP结合周期性机械牵拉对C2C12肌管萎缩的作用及机制,将实验分为CON组、TNF-α组、TNF-α+2%PRP组、TNF-α+2%PRP+Str组,并加入抑制剂,检测MuRF-1、Fbx32及Akt、p-Akt、p70、p-p70表达变化。结果:2%PRP组MyoD、MyoG表达含量明显增加;2%PRP+Str组MyoD、MyoG表达含量增加显著;萎缩实验中,改良吉姆萨染色显示,TNF-α组肌管明显萎缩,TNF-α+2%PRP组可改善肌管萎缩现象;TNF-α+2%PRP+Str组MuRF-1、Fbx32表达含量下降;使用抑制剂干预后,PRP结合周期性机械牵拉的抗萎缩作用被抑制。结论:2%PRP结合周期性机械牵拉可促进C2C12细胞分化,改善肌管萎缩,通过Akt/p70信号通路发挥作用。Objective:To study effects and mechanism of platelet-rich plasma combined with periodic mechanical stretch on C2C12 myotube atrophy.Method:The expression of MyoD and MyoG was detected by Western Blot(WB)to confirm the optimal concentration of PRP;Subsequently,the pro-differentiation effect of PRP and periodic mechanical stretch(Str)on C2C12 cells was explored;C2C12 cells were induced to shrink by TNF-α,and the models were verified by Modified Giemsa staining.To explore the effect and mechanism of 2%PRP combined with periodic mechanical stretching in improving the atrophy of C2C12 cells,the experiments were divided into control group,TNF-αgroup,TNF-α+2%PRP group,TNF-α+2%PRP+Str group.The changes of MuRF-1,Fbx32 and Akt,p-Akt,p70 and p-p70 were detected by adding the inhibitors.Result:The expression levels of MyoD and MyoG in the 2%PRP group and the 2%PRP+Str group were significantly increased.In the anti-atrophy experiment,the modified Giemsa staining showed the myotubes in the TNF-α group were significantly atrophied,and the TNF-α+2%PRP group can improve myotube atrophy.The expressions of MuRF-1 and Fbx32 were decreased in the TNF-α+2%PRP+Str group.After GDC0068 intervention,the anti-atrophic effect of PRP combined with periodic mechanical stretch was inhibited.Conclusion:2%PRP combined with periodic mechanical stretch can effectively promote the differentiation of C2C12 cells,improve myotube atrophy,and play a role through the Akt/p70 signaling pathway.

关 键 词：富血小板血浆 C2C12骨骼肌细胞 周期性机械牵拉 分化 肌肉萎缩 肌少症 

分 类 号：R493[医药卫生—康复医学] R681[医药卫生—临床医学]