不同时长运动预处理对血管性痴呆大鼠脑血管新生相关蛋白HIF-1α、VEGF、HO-1表达的影响  

Effects of exercise preconditioning with different duration on expressions of brain angiogenesis related proteins HIF-1α、VEGF and HO-1 in rats with vascular dementia

作　　者：陈婕 唐鑫[1] 张泓[1] 邹莹洁 谢紫薇 陈盼[1] 谢海花 谭洁[1] CHEN Jie;TANG Xin;ZHANG Hong(Hunan University of Chinese Medicine,Changsha,Hunan,410208)

机构地区：[1]湖南中医药大学针灸推拿与康复学院,湖南省长沙市410208

出　　处：《中国康复医学杂志》2025年第1期15-24,共10页Chinese Journal of Rehabilitation Medicine

基　　金：湖南省教育厅科学研究重点项目(22A0253);湖南中医药大学研究生创新课题(2023CX164);湖南中医药大学省级学科建设项目(中西医结合)(湘教通〔2022〕351号)。

摘　　要：目的:探讨不同时长运动预处理对血管性痴呆(vascular dementia,VD)大鼠脑血管新生相关蛋白HIF-1α、VEGF及HO-1表达的影响。方法:将SD雄性大鼠120只随机分为假手术组、模型组、运动预处理2周假手术组及模型组、运动预处理4周假手术组及模型组,每组各20只。运动预处理4周大鼠在造模前每周行5次不负重游泳训练30min,持续4周,而运动预处理2周大鼠持续2周。训练结束后,模型组、运动预处理2周、4周模型组行双侧颈总动脉结扎法制备VD模型,假手术组、运动预处理2周、4周假手术组只分离双侧颈总动脉不作结扎。造模后14d完成Morris水迷宫行为学检测,免疫组化法检测海马微血管密度、尼氏染色法检测海马神经元损伤情况、Western Blot法检测造模后3、7、14d海马中缺氧诱导因子-1α(hypoxia-inducible factorα,HIF-1α)、血管内皮生长因子(vascular endothelial growth factor,VEGF)及血红素加氧酶-1(heme oxygenase 1,HO-1)蛋白表达。结果:造模后14d,与假手术组比较,模型组逃避潜伏期延长、穿越平台次数减少(P<0.001,P=0.018);与模型组比较,运动预处理4周模型组逃避潜伏期减少(P=0.023)。与假手术组比较,模型组海马CA1区神经元数量减少(P<0.001);与模型组比较,运动预处理2周及4周模型组海马CA1区神经元数量增加(P=0.011,P<0.001);与运动预处理2周模型组比较,运动预处理4周模型组大鼠海马CA1区神经元数量增多(P=0.046)。与假手术组比较,模型组大鼠海马HIF-1α、VEGF及HO-1蛋白在造模后增加(P=0.013,P=0.037,P=0.044)。与模型组比较,运动预处理4周模型组大鼠海马HIF-1α、VEGF、HO-1蛋白在造模后增加(P=0.013,P=0.012,P=0.002)。与造模后3d及14d比较,VEGF蛋白在造模后7d表达升高(P=0.001,P<0.001);与造模后3d比较,HO-1蛋白在造模后7d表达升高(P=0.001)。与假手术组比较,模型组大鼠海马CA1区MVD增加(P=0.002);与模型组、运动预处理2周模型组比�Objective:To investigate the effects of exercise preconditioning with different durations on the expression of cerebral neovascularization related proteins HIF-1α,VEGF and HO-1 in rats with vascular dementia(VD).Method:One hundred and twenty SD male rats were randomly divided into sham group,model group,2-week exercise preconditioning sham and model group,4-week exercise preconditioning sham and model group,with 20 rats in each group.The 4-week exercise preconditioning rats received 30 minutes of nonweight-bearing swimming training 5 times a week for 4 weeks before modeling,while the 2-week exercise preconditioning rats lasted for 2 weeks.After training,in the model group,2-week and 4-week exercise preconditioning model groups,the bilateral carotid artery was ligated to establish the VD model,whereas in the sham group,2-week and 4-week exercise preconditioning sham group,only bilateral carotid artery was separated without ligation.At 14 days after modeling,Morris water maze was performed,immunohistochemical stain technique was used to detect the hippocampus microvessel density.Nissl's stain method was used to detect the damage of hippocampus neurons.Western Blot was used to detect the expression of hypoxia-inducible factorα(HIF-1α),vascular endothelial growth factor(VEGF)and heme oxygenase 1(HO-1)protein in the hippocampus of VD rats at 3,7 and 14 days after modeling.Result:At 14 days after modeling,compared with the sham group,the escape latency was prolonged and the times of crossing the platform was reduced in the model group(P<0.001,P=0.018).Compared with the model group,the escape latency of the 4-week exercise preconditioning model group was decreased(P=0.023).The number of neurons in hippocampal CA1 region decreased in the model group compared with the sham group(P<0.001).Compared with the model group,the number of neurons in hippocampal CA1 region in the 2-week and 4-week exercise preconditioning model groups increased(P=0.011,P<0.001).Compared with the 2-week exercise preconditioning model group,the n

关 键 词：血管性痴呆 运动预处理 血管新生 缺氧诱导因子-1Α 血管内皮生长因子 血红素加氧酶-1 

分 类 号：R493[医药卫生—康复医学]