骨伤宁膏治疗兔桡骨骨缺损实验研究  

Efficacy observation of Gushangning ointment on radius defect in rabbits

作　　者：马英锋[1] 贾戌生 兰志秀 李婷婷[1] 黑忠林[2] 张金玉 MA Ying-feng;JIA Xu-sheng;LAN Zhi-xiu(Ningxia Medical University,Yinchuan 750004,China;不详)

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏医科大学总医院 [3]宁夏回族自治区吴忠马莲渠张氏医院

出　　处：《山西中医》2025年第2期61-64,共4页Shanxi Journal of Traditional Chinese Medicine

基　　金：宁夏自然科学基金项目(编号:2020AAC03129,2022AAC03178)。

摘　　要：目的:观察骨伤宁膏对大白兔桡骨骨痂生长情况的影响,从血清生化指标与骨形态蛋白的表达、组织形态学等方面探讨张氏骨伤宁膏促进骨折愈合的作用机制。方法:建立大白兔骨缺损模型,随机分为模型组和骨伤宁膏组,每组各25只。在治疗第2、4、6和8周应用X射线观察骨折愈合过程,并检测血清中碱性磷酸酶(ALP)与钙(Ca)水平,使用苏木精-伊红染色观察治疗后两组大白兔桡骨恢复情况,使用免疫组化染色观察治疗后两组大白兔骨形态蛋白-2(BMP-2)的表达情况。结果:治疗第2、4、6和8周,骨伤宁膏组骨折部位X线评分及骨痂阴影面积均优于模型组(P﹤0.05)。治疗后,模型组骨折愈合不良,出现纤维性骨痂和骨髓腔梗阻;骨伤宁膏组骨折愈合良好,并恢复了正常结构,有效重塑了层状骨;骨伤宁膏组血清ALP与Ca水平、BMP-2表达均高于模型组(P﹤0.05)。结论:骨伤宁膏可通过上调BMP-2的表达,提高血清中碱性磷酸酶,增加钙含量,有效促进大白兔桡骨骨缺损愈合。Objective:To observe the effect of Gushangning ointment on the growth of radius callus in rabbits,and to approach the mechanism of Zhang's Gushangning ointment promoting fracture healing from serum biochemical indexes,bone morphogenetic protein expression and histomorphology.Methods:The models of radius defect in rabbit were established and randomly divided into model group and Gushangning ointment group,25 rabbits in each group.The fracture healing process was observed by X-ray at the 2nd,4th,6th and 8th week of treatment,and the levels of alkaline phosphatase(ALP)and calcium(Ca)in serum were detected.Rabbits'radius recovery was observed by Hematoxylin-eosin staining in two groups after treatment.The expression of bone morphogenetic protein-2(BMP-2)of rabbits was observed by immunohistochemical staining in two groups after treatment.Results:At the 2nd,4th,6th and 8th week of treatment,X-ray score of fracture site and shadow area of callus in Gushangning ointment group were better than those in model group(P﹤0.05).After treatment,the fracture healed badly in model group,and fibrous callus and marrow cavity obstruction appeared;the fracture healed well in Gushangning ointment group,and the normal structure was restored,the layered bone was effectively reshaped;serum ALP and Ca levels and BMP-2 expression in Gushangning ointment group were higher than those in model group(P﹤0.05).Conclusion:Gushangning ointment can increase alkaline phosphatase and calcium content in serum by up-regulating BMP-2 expression,and effectively promote the healing of rabbits'radius defect.

关 键 词：兔桡骨骨缺损 骨伤宁膏 实验研究 

分 类 号：R687.3[医药卫生—骨科学]