患红斑病中间球海胆肠道菌群结构和功能特征研究  

Structural and functional characteristics of intestinal bacterial community associated with red spotting disease of Strongylocentroyus intermedius

作　　者：陈东升 王荦[1,2,3] 邢腾宇 陈宇辰 陈新垚 丁君[1,2,3] CHEN Dongsheng;WANG Luo;XING Tengyu;CHEN Yuchen;CHEN Xinyao;DING Jun(Dalian Ocean University/Key Laboratory of Northern Aquatic Germplasm Resources and Genetic Breeding in Liaoning Province,Dalian 116023,China;Dalian Ocean University/Agriculture Department Key Laboratory of Mariculture&Stock Enhancement in North China Sea,Dalian 116023,China;Dalian Ocean University/Liaoning Province Aquatic Germplasm Resources Protection and Utilization Engineering Research Center,Dalian 116023,China)

机构地区：[1]大连海洋大学/辽宁省北方水产种质资源与遗传育种重点实验室,辽宁大连116023 [2]大连海洋大学/农业农村部北方海水增养殖重点实验室,辽宁大连116023 [3]大连海洋大学/辽宁省水产种质资源保护与利用工程研究中心,辽宁大连116023

出　　处：《南方水产科学》2025年第1期77-84,共8页South China Fisheries Science

基　　金：辽宁省科学技术厅农业重大专项项目(2023JH1/10200007);大连市重点研发计划项目(2022YF16SN067);国家重点研发计划“蓝色粮仓科技创新”项目(2018YFD0901604);辽宁省教育厅面上项目(LJKMZ20221105)。

摘　　要：红斑病是海胆养殖过程中最常见的细菌性疾病之一,具有发病速度快、传染性强、致死率高等特点。为揭示机体患病和肠道菌群间的相关性,基于16S rRNA测序技术,开展了患红斑病中间球海胆(Strongylocentroyus intermedius)肠道菌群结构和功能特征研究。结果显示,与健康组海胆相比,患病组海胆肠道菌群可操作分类单元(Operational taxonomic units,OTUs)数量减少,Alpha多样性显著下降(p<0.05)。门水平上,患病组海胆肠道菌群结构特征表现为变形菌门和拟杆菌门的相对丰度显著上升,以及厚壁菌门的相对丰度显著下降(p<0.05)。属水平上,Burkholderia_Caballeronia_Paraburkholderia、短波单胞菌属(Brevundimonas)、弧菌属(Vibrio)、假单胞菌属(Pseudomonas)等的相对丰度显著增加(p<0.05)。功能分析进一步揭示了患病组海胆肠道菌群代谢通路的变化,具体表现为肾素-血管紧张素系统、蛋白质消化、吸收、霍乱弧菌(V.cholerae)感染等相关代谢通路丰度显著增加,而叶酸生物合成途径通路丰度显著降低(p<0.05)。研究表明,红斑病扰乱了中间球海胆肠道微生态平衡,降低了肠道菌群的稳定性。Red spot disease is one of the most prevalent bacterial diseases in sea urchin(Strongylocentrotus intermedius)aquaculture,exhibiting rapid development,high contagion and a considerable mortality rate.We investigated the gut bacterial community composition and function characteristics of sea urchins with red spotting disease by using 16S rRNA sequencing technology,so as to reveal the correlation between diseases and gut bacterial communities.The results demonstrate that com-pared with the healthy sea urchins,the number of operational taxonomic units(OTUs)and the Alpha index in the gut microbiota of the diseased ones decreased significantly(p<0.05).On phylum level,the relative abundance of Proteobacteria and Bacteroi-dota in the gut of diseased sea urchin increased significantly,while the relative abundance of Firmicutes was significantly lower(p<0.05).On genus level,the relative abundance of Burkholderia_Caballeronia_Paraburkholderia,Brevundimonas,Vibrio and Pseudomonas increased significantly(p<0.05).The analysis of the functional characteristics of the microbial communities reveals that the paths related to the Renin-angiotensin system,protein digestion,and Vibrio cholerae infection in diseased sea urchins increased significantly,while the Folate biosynthesis pathway decreased significantly(p<0.05).The results indicate that the microbial ecological balance and stability were reduced by red spot disease in the gut bacterial community of S.intermedius.

关 键 词：中间球海胆 红斑病 16SrRNA 肠道菌群 

分 类 号：S966.9[农业科学—水产养殖]