横带髭鲷早期发育阶段消化系统组织学观察  

Organogenesis of digestive system in Hapalogenys mucronatus during early development

作　　者：平洪领 付铁中 张涛[1,2] 史会来 周群[2] 邹京华 PING Hongling;FU Tiezhong;ZHANG Tao;SHI Huilai;ZHOU Qun;ZOU Jinghua(Zhejiang Marine Fisheries Research Institute/Zhejiang Province Key Laboratory of Mariculture and Enhancement,Zhoushan 316021,China;Zhejiang Ocean University/Zhejiang Marine Fisheries Research Institute,Zhoushan 316021,China)

机构地区：[1]浙江省海洋水产研究所/浙江省海水增养殖重点实验室,浙江舟山316021 [2]浙江海洋大学海洋与渔业研究所,浙江舟山316021

出　　处：《南方水产科学》2025年第1期164-172,共9页South China Fisheries Science

基　　金：浙江省自然科学基金项目(LTGN24C190010);浙江省重点研发计划项目(2023C02050);浙江省“三农九方”科技协作计划项目(2022SNJF073);浙江省海洋水产研究所科技计划项目(HYS-CZ-202317,HYS-CZ-202407);舟山渔业育种育苗科创中心专项(2023Y001-4)。

摘　　要：了解横带髭鲷(Hapalogenys mucronatus)仔、稚、幼鱼消化系统的形态组织学特征,可为其发育生物学、消化生理及投饵策略提供理论依据。通过形态学观察与连续组织切片及HE染色技术,对横带髭鲷0~45 d仔、稚、幼鱼消化系统的形态组织进行观察。结果表明:在水温(24±2)℃条件下,0~2 d为内源性营养期,口咽腔、食道和胃已贯通;3~8 d为混合营养期,肝脏、胰脏细胞团已形成,可以摄食轮虫,口咽腔中产生黏液细胞和味蕾,食道及肠形成黏膜褶皱,胰脏出现酶原颗粒,此阶段仔鱼已具备初步的摄食消化能力,可以投喂轮虫等饵料;9 d之后为外源性营养期,肠道弯曲,黏膜褶皱丰富,纹状缘发达,胃分为贲门区、胃体区和幽门区,胃腺和胃小凹也形成,肝脏肝血窦数量多,胰脏可见酶原颗粒,此阶段可摄食一些蒙古裸腹溞(Moina mongolica)以及大的桡足类;22~30 d各器官进一步发育;35 d消化系统结构和功能基本完善。横带髭鲷早期发育阶段消化系统发育特征与其摄食方式及功能密切相关。Understanding the morphological and histological characteristics of the digestive system of larvae,juveniles and young fish of Hapalogenys mucronatus can provide a theoretical basis for its developmental biology,digestive physiology and feeding strategy.Through morphological observation,continuous tissue sectioning and HE staining techniques,we examined histologically the development of the digestive system of the larvae,juveniles and young fish of H.mucronatus from 0th-45th day after hatching.The results show that at the water temperature of(24±2)℃,0-2 day was the endogenous nutritional period,and the oropharyngeal cavity,esophagus and stomach had been penetrated.During the mixed nutrition period of 3-8 day,the cell masses of liver and pancreas had been formed,possible to feed on rotifers.Mmucus cells and taste buds were produced in the oropharyngeal cavity,mucosal folds were formed in the esophagus and intestine,and zymogen particles appeared in the pan-creas.At this stage,the larvae had preliminary feeding and digestive abilities,and could be fed with rotifers and other bait.After 9 days of age,it was the exogenous nutritional period,with a curved intestinal tract,abundant mucosal folds and well-developed striated margins.The stomach was divided into a cardiac region,a gastric body and a pyloric region.Gastric glands and gastric pits were also formed,with a large number of hepatic sinuses,and zymogen particles visible in the pancreas.At this stage,it was possible to ingest some Moina mongolica and large copepods.From 22 to 30 days,each organ further developed,and after 35 d,the digestive system structure and function were basically improved.The development characteristics of digestive system at the early development stage of H.mucronatus are closely related to its feeding mode and function.

关 键 词：横带髭鲷 消化系统 器官发生 组织学 

分 类 号：S917.4[农业科学—水产科学]