轮状病毒感染相关腹泻患儿肠道菌群特征与血清锌、肌酸激酶同工酶及炎症因子的关系  

Relationship between intestinal flora characteristics and serum zinc,CK-MB and inflammatory factors in children with rotavirus infection-related diarrhea

作　　者：杜宝静 李敏叶 王云霞 申永静 谈威威 Du Baojing;Li Minye;Wang Yunxia;Shen Yongjing;Tan Weiwei(Department of Pediatric,Handan Maternal and Child Health Hospital,Handan 056001,China)

机构地区：[1]邯郸市妇幼保健院儿科,河北省056001

出　　处：《中华消化病与影像杂志(电子版)》2025年第1期78-82,共5页Chinese Journal of Digestion and Medical Imageology(Electronic Edition)

基　　金：河北省卫生和计划生育委员会制(20191809)。

摘　　要：目的分析轮状病毒感染相关腹泻患儿肠道菌群特征与血清锌、肌酸激酶同工酶(CK-MB)及炎症因子的关系。方法选取2018年1月至2023年12月邯郸市妇幼保健院收治的200例轮状病毒感染相关腹泻患儿,按照其病情将患儿分别纳入轻度组(n=141)、中重度组(n=59),并选取同期80例非感染性腹泻患儿,纳入对照组。使用16SrRNA荧光定量聚合酶链式反应(PCR)技术检测各组患儿粪便双歧杆菌、大肠杆菌、乳酸杆菌含量,并检测其血清锌、CK-MB、炎症因子水平。分析轮状病毒感染相关腹泻患儿肠道菌群特征,并使用Pearson相关性分析,计算粪便肠道菌群含量与血清锌、CK-MB、炎症因子的相关性。结果轻度组患儿粪便双歧杆菌、乳酸杆菌含量较对照组下降,中重度组患儿粪便双歧杆菌、乳酸杆菌含量较轻度组下降(P<0.05)。轻度组患儿血清锌较对照组下降、CK-MB较对照组升高,中重度组患儿血清锌较轻度组下降、CK-MB较轻度组升高,差异均有统计学意义(P<0.05)。轻度组患儿血清IFN-γ、IL-6、IL-10、TNF-α均较对照组升高,中重度组患儿IFN-γ、IL-6、IL-10、TNF-α均较轻度组升高(P<0.05)。Pearson相关性分析示,轮状病毒感染相关腹泻患儿双歧杆菌、乳酸杆菌含量均与血清锌含量呈正相关,与CK-MB、IFN-γ、IL-6、IL-10、TNF-α含量呈负相关(P<0.05)。结论轮状病毒感染相关腹泻患儿肠道菌群以双歧杆菌、乳酸杆菌含量下降为主要特征,随着病情进展双歧杆菌、乳酸杆菌含量下降愈发显著,这一变化伴随着血清锌含量的下降及CK-MB、炎症因子含量的上升。Objective To analyze the relationship between the characteristics of intestinal flora and serum zinc,creatine kinase isoenzyme(CK-MB)and inflammatory factors in children with rotavirus infection-related diarrhea.Methods A total of 200 children with rotavirus infection-related diarrhea admitted to Handan Maternal and Child Health Hospital from January 2018 to December 2023 were divided into mild group(n=141)and moderate severe group(n=59)according to their condition,and 80 children with non-infectious diarrhea were selected and included in the control group.16SrRNA fluorescence quantitative polymerase chain reaction(PCR)was used to detect the contents of Bifidobacterium,Escherichia coli and Lactobacillus in feces of children in each group,and the serum zinc,CK-MB and inflammatory factors were detected.The characteristics of intestinal flora in children with rotavirus infection-related diarrhea were analyzed,and Pearson correlation analysis was used to calculate the correlation between fecal intestinal flora content and serum zinc,CK-MB and inflammatory factors.Results The contents of Bifidobacterium and Lactobacillus in feces of children in mild group were lower than those in control group,and those in moderate and severe group were lower than those in mild group(P<0.05).The serum zinc content in mild group was lower than that in control group,and CK-MB was higher than that in control group.The serum zinc content in moderate and severe group was lower than that in mild group,and CK-MB was higher than that in mild group,with statistical significance(P<0.05).The levels of serum IFN-γ,IL-6,IL-10 and TNF-αin mild group were higher than those in control group,and the levels of serum IFN-γ,IL-6,IL-10 and TNF-αin moderate and severe group were higher than those in mild group(P<0.05).Pearson correlation analysis showed that the contents of Bifidobacterium and Lactobacillus in children with rotavirus infection-related diarrhea were positively correlated with serum zinc content,and negatively correlated with CK-MB,IFN-�

关 键 词：轮状病毒感染 腹泻 肠道菌群 微量元素 肌酸激酶同工酶 炎症因子 

分 类 号：R725.7[医药卫生—儿科]